Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

Nadine Alvarez; Daymí Serpa; Yanely Tirado; Reinier Borrero; Sonsire Fernández; Rubén Cabrera; Yolanda Valdes; Caridad Zayas; Reinaldo Acevedo; Luis Izquierdo; José Luis Pérez; Ramlah Kadir; María Elena Sarmiento; Mohd-nor Norazmi; Mohd-nor Norazmi; Armando Acosta

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Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

Author: Nadine ALVAREZ ¹ ; Daymí SERPA ¹ ; Yanely TIRADO ¹ ; Reinier BORRERO ¹ ; Sonsire FERNÁNDEZ ¹ ; Rubén CABRERA ¹ ; Yolanda VALDES ¹ ; Caridad ZAYAS ¹ ; Reinaldo ACEVEDO ¹ ; Luis IZQUIERDO ¹ ; José Luis PÉREZ ¹ ; Ramlah KADIR ² ; María Elena SARMIENTO ² ; Mohd-Nor NORAZMI ² ; Mohd-Nor NORAZMI ³ ; Armando ACOSTA ³
Author Information

1. Finlay Institute
2. School of Health Sciences, University Sains Malaysia
3. Institute for Research in Molecular Medicine, University Sains Malaysia
Publication Type:Journal Article
Keywords: BCG; Immunogenicity; Mycobacterium tuberculosis; Proteoliposomes
From:Asian Pacific Journal of Tropical Biomedicine 2017;7(3):188-192
CountryChina
Language:Chinese
Abstract: Objective To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis (M. tuberculosis) of a proteoliposome (PL) from Mycobacterium bovis Bacillus Calmette–Guérin (BCG) with and without alum hydroxide (AL) as adjuvant (PLBCG-AL and PLBCG, respectively) in BALB/c mice. Methods BALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immunoglobulin G (IgG), IgG1 and IgG2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western blot. Results Sera from animals immunized with PLBCG-AL and PLBCG showed significant increase in specific total IgG and IgG1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in vivo. Conclusions The cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the protective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages.