Generation of antibodies against disintegrin and cysteine-rich domains by DNA immunization: An approach to neutralize snake venom-induced haemorrhage
10.1016/j.apjtb.2016.12.015
- Author:
Sidgi Syed Anwer Abdo HASSON
1
Author Information
1. Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University
- Publication Type:Journal Article
- Keywords:
Antibody zymography;
Antivenoms;
DNA-immunization;
Echis ocellatus;
GeneGun;
Neutralization;
Snake
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(3):198-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether a DNA immunization approach targeting the major haemorrhage molecule of a prothrombin activator-like metalloproteinase from Echis ocellatus (E. ocellatus) venom could be conceived to inspire antibodies with more prominent specificity and equal adequacy to current conventional antivenoms systems. Methods The isolated DNA EoMP-6 was used as the template for PCR amplification using the EoDC-2-specific forward and reverse primers. A PCR product of approximately 700 bp was obtained and cloned into pSecTag-B expression vector where anti-EoDC-2 antibodies were generated and analysed for their efficacy to neutralise local haemorrhage in vitro and in vivo. Results Our results suggest that the generated anti-EoDC-2 showed a remarkable efficacy by (a) interfering with the interaction of the recombinant disintegrin “EoDC-2” isolated from the E. ocellatus as well as other viper species to the α
