Antimicrobial activity of crude extracts prepared from fungal mycelia
10.1016/j.apjtb.2016.12.011
- Author:
Andriy SYNYTSYA
1
;
Roman BLEHA
1
;
Jutamart MONKAI
2
;
Ekachai CHUKEATIROTE
2
;
Anna MACURKOVA
3
;
Tomas RUML
3
;
Juhee AHN
4
Author Information
1. Department of Carbohydrates and Cereals, University of Chemistry and Technology
2. School of Science, Mae Fah Luang University
3. Department of Biochemistry and Microbiology, University of Chemistry and Technology
4. Department of Medical Biomaterials Engineering, Kangwon National University
- Publication Type:Journal Article
- Keywords:
Antimicrobial activity;
Disc diffusion;
Fungal mycelia;
Partitioned extracts
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(3):257-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the in vitro antimicrobial property of three different partitioned extracts (petroleum ether, ethanol and water) prepared from some fungal mycelia. Methods Seven fungal mycelia were prepared, initially extracted with acidified ethanol (0.2 mol/L HCl in 80% ethanol), yielding the raw crude extracts. The obtained extracts were then further partitioned with petroleum ether (F1), ethanol (F2) and water (F3). All the fractions were tested for antimicrobial activity using the disc diffusion assay. Results Our data showed that all the fractions could inhibit the testing bacteria. However, the inhibitory activity was found to be dependent on (i) the fungal strains used; (ii) the solvent extracted; and (iii) the testing bacteria assayed. In general, the ethanolic extracts (F2) derived from all fungi displayed highest inhibitory activity against the testing bacteria except for Chaetomium sp. Conclusions The findings of the present study concluded that the extracts prepared from the fungal mycelia had the bioactive compounds with antibacterial property. This study is a pioneering work and further study should be carried out for development of the new drug leads.
