Prevalence and virulence factors of Candida spp. associated with blow flies

Wimonrat GUNTANG; Nathamon KAMONVORADEJ; Chitchanok CHOMCHAT; Jintana WONGWIGKARN; Nophawan BUNCHU; Damrongpan THONGWAT; Supaporn LAMLERTTHON; Chitchanok CHOMCHAT; Sangrawee SURIYAKAN; Jintana WONGWIGKARN; Nophawan BUNCHU; Damrongpan THONGWAT; Supaporn LAMLERTTHON; Sangob SANIT

Return

Prevalence and virulence factors of Candida spp. associated with blow flies

Author: Wimonrat GUNTANG ¹ ; Nathamon KAMONVORADEJ ¹ ; Chitchanok CHOMCHAT ¹ ; Jintana WONGWIGKARN ¹ ; Nophawan BUNCHU ¹ ; Damrongpan THONGWAT ¹ ; Supaporn LAMLERTTHON ¹ ; Chitchanok CHOMCHAT ² ; Sangrawee SURIYAKAN ² ; Jintana WONGWIGKARN ² ; Nophawan BUNCHU ² ; Damrongpan THONGWAT ² ; Supaporn LAMLERTTHON ² ; Sangob SANIT ³
Author Information

1. Centre of Excellence in Fungal Research, Faculty of Medical Science, Naresuan University
2. Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University
3. Department of Parasitology, Faculty of Medicine, Chiang Mai University
Publication Type:Journal Article
Keywords: Antifungal resistance; Blow flies; Candida albicans; Phospholipase; β-Hemolysin
From:Asian Pacific Journal of Tropical Biomedicine 2017;7(5):428-431
CountryChina
Language:Chinese
Abstract: Objective To investigate the prevalence of Candida spp. and the virulence factors of Candida albicans (C. albicans) isolated from external surfaces of blow flies collected from Mae Sot, Tak Province, Thailand. Methods The blow flies were collected by sterile sweep nets from three areas in Mae Sot. Yeast isolation was first performed on Sabouraud dextrose agar (SDA) supplemented with chloramphenicol and cycloheximide. The yeast isolates were then identified by using chromogenic agar, a yeast identification test kit, a germ tube formation test and a carbohydrate utilization test. The β-hemolysis was determined on 7% sheep blood agar, while phospholipase activity was measured on SDA agar supplemented with 10% egg yolk suspension. Antifungal susceptibility testing was determined by broth micro-dilution testing against ketoconazole and amphotericin B. Results The prevalence rate of Candida spp. on the external surfaces of the blow flies was 78.1%. All C. albicans isolated from the blow fly demonstrated β-hemolysin and potent phospholipase activities and 47.1% of C. albicans were resistant to ketoconazole with MIC values 128 μg/mL. Conclusions The results indicate that blow flies could play an essential role in the transmission of potentially pathogenic and antifungal resistant C. albicans into the environment. Further investigation on other virulence factors and genetic relatedness among isolates from the blow flies, the environment and clinical specimens is required to confirm this role.