The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats
10.1016/j.apjtb.2017.06.013
- Author:
Volkan GELEN
1
;
Gözde ATILA
1
;
Mustafa MAKAV
1
;
Emin ŞENGÜL
2
;
Semin GEDIKLI
3
;
Hamit USLU
4
Author Information
1. Department of Physiology, Veterinary Faculty, Kafkas University
2. Department of Physiology, Veterinary Faculty, Atatürk University
3. Department of Histology-Embryology, Veterinary Faculty, Atatürk University
4. Department of Health Care Services, Atatürk Health Vocational School, Kafkas University
- Publication Type:Journal Article
- Keywords:
5-Fluorouracil;
Hepatotoxicity;
Quercetin;
Rat;
Rutin
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(7):647-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of quercetin (Q) and rutin on 5-fluorouracil (5-FU)-induced hepatotoxicity. Methods The control group was corn oil. The 5-FU group rats were corn oil and injected intraperitoneal 5-FU 50 mg/kg. Groups rutin 50 + 5-FU and rutin 100 + 5-FU were respectively 50 mg/kg and 100 mg/kg rutin. These groups were given 5-FU (50 mg/kg) in the 18th day. The group rutin 100 was rutin (100 mg/kg i.g.). Groups Q50 + 5-FU and Q100 + 5-FU were respectively 50 mg/kg and 100 mg/kg quercetin. These groups were given 5-FU (50 mg/kg) in the 18th day of quercetin application. The group Q100 was quercetin (100 mg/kg i.g.). In the end of experimental applications, blood was collected from anesthetized rats. Results The MDA level was significantly higher in the 5-FU group compared with control group, and determined to be decreased in other groups. GPx and GSH levels were significantly decreased in the 5-FU group compared to the control, rutin 100 + 5-FU and Q100 + 5-FU groups. AST, ALT, LDH and ALP levels in the serum were significantly increased in the 5-FU group compared with the other groups. The results from this analysis show that while the caspase-3 level increases in the 5-FU group, it decreases in the Q50 + 5-FU, Q100 + 5-FU, rutin 50 + 5-FU and rutin 100 + 5-FU groups. Bcl-2 level decreased in the 5-FU group compared to the control group, but increased in the rutin 100 + 5-FU, Q50 + 5-FU and Q100 + 5-FU groups. Conclusions In this study it was determined that the rutin and Q have protective effects on 5-FU-induced hepatotoxicity.
