Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus
- Author:
Achmad Fuad HAFID
1
;
Adita Ayu PERMANASARI
1
;
Myrna ADIANTI
1
;
Lydia TUMEWU
1
;
Aty WIDYAWARUYANTI
1
;
Tutik Sri WAHYUNI
1
;
Maria Inge LUSIDA
1
;
Maria Inge SOETJIPTO
1
;
Achmad Fuad HAFID
2
;
Aty WIDYAWARUYANTI
2
;
Tutik Sri WAHYUNI
2
;
Chie AOKI-UTSUBO
3
;
Maria Inge LUSIDA
4
;
Sri Puji Astuti WAHYUNINGSIH
5
;
Hak HOTTA
6
Author Information
- Collective Name:Department of Biochemistry, Faculty of Medicine, Airlangga University
- Publication Type:Journal Article
- Keywords: Antiviral; Artocarpus heterophyllus; Artocarpus sp.; Hepatitis C virus
- From:Asian Pacific Journal of Tropical Biomedicine 2017;7(7):633-639
- CountryChina
- Language:Chinese
- Abstract: Objective To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti-HCV activity with an inhibitory concentration (IC
