An overview on application of phage display technique in immunological studies
10.1016/j.apjtb.2017.06.001
- Author:
Abbas RAMI
1
;
Mahdi BEHDANI
1
;
Fatemeh KAZEMI-LOMEDASHT
1
;
Najmeh YARDEHNAVI
2
;
Najmeh YARDEHNAVI
3
;
Mahdi HABIBI-ANBOUHI
4
Author Information
1. Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab., Pasteur Institute of Iran
2. Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences
3. Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences
4. National Cell Bank of Iran, Pasteur Institute of Iran
- Publication Type:Journal Article
- Keywords:
Antibody;
Antibody library;
Epitope mapping;
Immunology;
Peptide library;
Phage display
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(7):599-602
- CountryChina
- Language:Chinese
-
Abstract:
Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and isolated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.
