Chemical profile and antimicrobial activity of Secondatia floribunda A. DC (Apocynaceae)
10.1016/j.apjtb.2017.07.009
- Author:
Daiany Alves RIBEIRO
1
;
Irwin Rose Alencar DE MENEZES
1
;
Marta Maria de Almeida SOUZA
1
;
José Galberto Martins DA COSTA
1
;
Daiany Alves RIBEIRO
2
;
Sarah Soares DAMASCENO
2
;
José Galberto Martins DA COSTA
2
;
Aline Augusti BOLIGON
3
;
Marta Maria de Almeida SOUZA
4
;
Irwin Rose Alencar DE MENEZES
5
Author Information
1. Programa de Pós-Graduação em Etnobiologia e Conservação da Natureza, Universidade Regional do Cariri
2. Departamento de Química Biológica, Laboratório de Pesquisas de Produtos Naturais, Universidade Regional do Cariri
3. Laboratório de Fitoquímica, Universidade Federal de Santa Maria
4. Departamento de Ciências Biológicas, Laboratório de Ecologia Vegetal, Universidade Regional do Cariri
5. Departamento de Química Biológica, Laboratório de Farmacológia e Química Molecular, Universidade Regional do Cariri
- Publication Type:Journal Article
- Keywords:
Chemical composition;
Cinchonain;
HPLC;
Modification resistance;
Natural product
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(8):739-749
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the chemical profile, and to evaluate the antibacterial and modulatory activity of the ethanolic extracts of the stalk's inner bark and heartwood of Secondatia floribunda. Methods Quantification of total phenols and flavonoids was determined by the Folin-Ciocalteu method and aluminum chloride, respectively. Phenolic compounds were identified and quantified by HPLC-DAD (High Performance Liquid Chromatography-Diodearray Detector) and the Infrared Spectroscopy was performed using the measure by Attenuated Total Reflectance with Fourier Transform (ATR-FTIR). Antibacterial assays for determination of the Minimum Inhibitory Concentration (MIC) and modification of aminoglycosides were performed by microdilution. Results Infrared spectra showed similar characteristics, having among its main absorption bands hydroxyl group (OH). The antibacterial activity showed clinically significant results for the strains of Staphylococcus aureus and Escherichia coli. In modulation assay, synergic and antagonistic effect for both extracts was observed. Heartwood extract in combination with antibiotics showed a significant MIC reduction at 19.8% (P < 0.000 1)-79.3% (P < 0.01). Conclusions This study is the first report of chemical and biological information of Secondatia floribunda suggesting that it is clinically relevant source of a new antibacterial therapy, especially due to the presence of significant levels of phenolic compounds.