Rourea cuspidata: Chemical composition and hypoglycemic activity
10.1016/j.apjtb.2017.07.015
- Author:
Manuela M. LAIKOWSKI
1
;
Paulo R. DOS SANTOS
1
;
Debora M. SOUZA
1
;
Luciane MINETTO
1
;
Leandro TASSO
1
;
Sidnei MOURA
1
;
Natalia GIRONDI
2
;
Camila PIRES
2
;
Gisiele ALANO
2
;
Leandro TASSO
2
;
Mariana ROESCH-ELY
3
Author Information
1. Laboratory of Natural and Synthetics Products, University of Caxias do Sul
2. Laboratory of Pharmacology, University of Caxias do Sul
3. Laboratory of Genomics, Proteomics and DNA Repair, University of Caxias do Sul
- Publication Type:Journal Article
- Keywords:
Diabetes;
HRMS;
Phytochemical characterization;
Rourea cuspidata
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(8):712-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antidiabetic effect of Rourea cuspidata hydroalcoholic stem extract in normal and streptozotocin-induced diabetic rats. Methods In order to evaluate the chemical composition, different extracts from stem in ascending solvent order of polarity were prepared. The extracts were analyzed by high resolution mass spectrometry and 7 compounds were identified, including hyperin, an important and already reported active compound in the literature. Hyperin was also quantified by HPLC-UV in all the extracts. The hydroalcoholic stem extract (Ss5), which showed the highest concentration of hyperin, was administered to STZ-induced diabetes rats to evaluate the potential hypoglycemic activity. Total cholesterol, HDL, triglycerides, ALT and AST were also evaluated. In the present study, the effects of oral administration of hydroalcoholic stem extract (200 mg/kg b. wt.) for 28 days on the level of serum glucose, total cholesterol, HDL, triglycerides, aspartate amino transferase (AST) and alanine amino transferase (ALT) in normal and streptozotocin-induced diabetic rats were evaluated. Histopathological changes in diabetic rats' pancreas were also studied. Results The extract exposition demonstrated hypoglycemic effect like the drug control glibenclamide. The extract was able to increase the HDL levels. Histopathological study on diabetic rats' pancreas after extract treatment showed morphological alterations in STZ-induced diabetes rats, which were apparently restored after extract treatment. Conclusions This work demonstrates the potential use of R. cuspidata as hypoglycemic agent in the treatment of diabetes.
