LC–MS characterization, anti-kinetoplastide and cytotoxic activities of natural products from Eugenia jambolana Lam. and Eugenia uniflora
10.1016/j.apjtb.2017.08.007
- Author:
Celestina Elba SOBRAL DE SOUZA
1
;
Ana Raquel PEREIRA DA SILVA
1
;
Janaina Esmeraldo ROCHA
1
;
Henrique Douglas Melo COUTINHO
1
;
Maria Celeste VEGA GOMEZ
2
;
Míriam ROLÓM
2
;
Cathia CORONEL
2
;
José Galberto MARTINS DA COSTA
3
;
Manoel L.C. NETTO
4
;
Larissa A. ROLIM
5
Author Information
1. Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri
2. Center for the Development of Scientific Research, Moisés Bertoni Foundation/Díaz Gill Laboratories
3. Laboratório de Pesquisa de Produtos Naturais, Universidade Regional do Cariri
4. Centro Universitário Dr. Leão Sampaio – UNILEÃO
5. Universidade Federal do Vale do São Francisco – UNIVASF
- Publication Type:Journal Article
- Keywords:
Antiparasitic effect;
Cytotoxicity;
Eugenia jambolana;
Eugenia uniflora
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(9):836-841
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the trypanocidal, leishmanicidal and cytotoxic activity of Eugenia jambolana (E. jambolana) and Eugenia uniflora (E. uniflora) extracts and fractions. Methods The products were characterized by LC–MS. Antiparasitic assays were performed and cytotoxicity was evaluated in fibroblastos. In vitro assays were performed using spectrophotometric evaluation. All assays were performed in thrice. Results The results showed that the extracts and the tannic fraction from E. jambolana inhibited 100% of the epimastigote lines. The ethanolic extract was the most efficient in all concentrations tested against the three parasite strains. In the cytotoxicity assay the flavonoid fraction showed low toxicity. All E. uniflora samples showed cytotoxicity at the highest concentration tested, but the extract showed no toxic effect on the fibroblasts at the lowest concentration. The flavonoid and tannic fractions were more efficient against Leishmania braziliensis promastigotes compared to the extract. However, the extracts and the tannic fraction were more effective against Leishmania infantum strains. The effect on epimastigote cells was observed at all concentrations tested, with all E. uniflora samples. However, the samples were more effective at the highest concentration, where there was inhibition in 100% of the Trypanosoma cruzi strains. Conclusions The species E. jambolana and E. uniflora presented antiparasitic activity against all tested parasite strains, indicating that these species can serve as an alternative therapy as they were efficient in the tests performed. The E. uniflora extract and the E. jambolana flavonoid fraction presented a low cytotoxicity, opening the floor for new biological studies.
