Comparison of electrochemiluminescence and ELISA methods in the detection of blood borne pathogens in Gabon
10.1016/j.apjtb.2017.08.008
- Author:
Cyrille BISSEYE
1
;
Anicet Mouity MATOUMBA
1
;
Bolni Marius NAGALO
1
;
Jophrette Mireille Ntsame NDONG
2
;
Calixte BENGONE
2
;
Guy Mouelet MIGOLET
2
;
Bolni Marius NAGALO
3
Author Information
1. Laboratory of Molecular and Cellular Biology, University of Sciences and Techniques of Masuku (USTM)
2. National Blood Transfusion Center
3. Department of Hematology/Oncology, Mayo Clinic Scottsdale
- Publication Type:Journal Article
- Keywords:
Blood donors;
Cobas 6000 e601;
EVOLIS BioRad;
Kappa coefficient
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(9):805-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the performances of Cobas 6000 e601 and EVOLIS BioRad in the detection of HIV, HBV and HCV in blood donors in Libreville (Gabon). Methods A cross-sectional investigation was conducted in July 2017 in a total of 2 000 blood donors recruited at the National Blood transfusion Center, Libreville Gabon. Among them, 363 donors were selected to compare the performances of COBAS 6000 e601 (electro-chemiluminescence) and EVOLIS BioRad in detecting HIV, HBV and HCV using Cohen's kappa coefficient. Results Both methods yielded similar results for the detection of HIV and HBsAg. A very good agreement of 93.39% and an excellent agreement of 98.90% were obtained for the detection of HIV and HbsAg, with kappa values of 0.80 and 0.98, respectively. The observed agreement of 91.86% was found for the detection of HCV, which gave a fair agreement between the two methods with kappa = 0.33. Conclusions The two evaluation methods showed a similar performance in the detection of HIV, HBV. However, given the high rate of intra and inter-genotypes recombination known for HIV and HBV, more robust techniques of detection such as polymerase chain reaction should be used to prevent post-transfusion contaminations.
