Isolation of Stenotrophomonas maltophilia from clinical samples: An investigation of patterns motility and production of melanin pigment
10.1016/j.apjtb.2017.08.012
- Author:
Rabeeh Izadi AMOLI
1
;
Azar SABOKBAR
1
;
Jamileh NOWROOZI
2
;
Ramazan RAJABNIYA
3
Author Information
1. Department of Microbiology, Karaj Branch, Islamic Azad University
2. Microbiology Department of Islamic Azad University North Tehran Branch
3. Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
23S rRNA;
Melanin;
Motility;
Stenotrophomonas maltophilia
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(9):826-830
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate possible sources of Stenotrophomonas maltophilia (S. maltophilia) in the clinical environment. Methods Different samples were collected from Amol City of Iran. Steps for the identification of S. maltophilia included culturing, biochemical tests, polymerase chain reaction (PCR) of 16S rRNA gene and 23S rRNA gene. In addition, production of melanin pigment and patterns of motility of the bacteria, were also investigated. Results In our study, 20 S. maltophilia strains were isolated from clinical sources, oxygen manometer apparatus of hospitals were 7/110 (6.36%), blood was 1/777 (0.13%), sputum was 4/40 (4%), urine was 1/2 947 (0.03%), tap water was 1/240 (0.42%) and dental suction was 6/120 (5%). The isolated bacteria showed production of melanin pigment with rates of strong, moderate, weak, and lack of pigment. Types of motilities were seen in isolates. Conclusions The highest percentage of bacteria is isolated of oxygen manometer system and dental suction, yet has not been reported from oxygen manometer system. These bacteria have also been associated with patients who have respiratory problems, so it is essential for staffs of hospitals to draw attention to this source of bacteria.
