An update on Gardneralla vaginalis associated bacterial vaginosis in Malaysia
10.1016/j.apjtb.2017.08.011
- Author:
Nada Khairi YOUNUS
1
;
Syafinaz Amin NORDIN
1
;
Narcisse MARY
1
;
Vasantha Kumari NEELA
1
;
Renu GOPINATH
2
;
Ravindran JEGASOTHY
2
;
Alex VAN BELKUM
3
Author Information
1. Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2. Obstetrics and Gynaecology, MAHSA University College
3. bioMérieux, R&D Microbiology, La Balme les Grottes
- Publication Type:Journal Article
- Keywords:
ARDRA;
Gardnerella vaginalis;
Piot's biotype;
Sialidase;
Vaginolysin
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(9):831-835
- CountryChina
- Language:Chinese
-
Abstract:
Objective To update the status of Gardnerella vaginalis (G. vaginalis) as a causative agent of bacterial vaginosis (BV) in Malaysia and to define its epidemiology, metronidazole resistance and virulence properties. Methods It is a single-centre (Gynaecology clinic at the Hospital Kuala Lumpur, Malaysia) prospective study with laboratory-based microbiological follow up and analyses. Vaginal swabs collected from the patients suspected for BV were subjected to clinical BV diagnosis, isolation and identification of G. vaginalis, metronidazole susceptibility testing, vaginolysin and sialidase gene PCR, Piot's biotyping and amplified ribosomal DNA restriction analysis (ARDRA) genotyping. Results Among the 207 patients suspected for BV, G. vaginalis was isolated from 47 subjects. G. vaginalis coexisted with Trichomonas vaginalis and Candida albicans in 26 samples. Three G. vaginalis isolates were resistant to metronidazole. Biotyping revealed 1 and 7 as the common types. ARDRA genotype II was found to be more common (n = 22; 46%) than I (n = 12; 25.53%) and III (n = 13; 27.6%). All genotype I and III isolates carried the sialidase gene, while 91.6% and 84.6% contained the vaginolysin gene. Genotype I was significantly associated with post-gynaecological surgical complications and abortions (P = 0.002). Conclusions The existence of pathogenic G. vaginalis clones in Malaysia including drug resistant strains should not be taken lightly and needs to be monitored as these may bring more complications especially among women of child bearing age and pregnant women.
