Anti-inflammatory properties of oolong tea (Camellia sinensis) ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells
10.1016/j.apjtb.2017.10.002
- Author:
Arina NOVILLA
1
;
Dedi Somantri DJAMHURI
1
;
Betty NURHAYATI
2
;
Dwi Davidson RIHIBIHA
3
;
Ervi AFIFAH
3
;
Wahyu WIDOWATI
4
Author Information
1. School of Health Sciences Jenderal Achmad Yani Cimahi
2. Department of Health Analyst, Health Sciences Polytechnic Bandung
3. Biomolecular and Biomedical Research Center, Aretha Medika Utama
4. Medical Research Center, Faculty of Medicine, Maranatha Christian University
- Publication Type:Journal Article
- Keywords:
Camellia sinensis;
EGCG;
Inflammation;
Macrophages
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(11):1005-1009
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-inflammatory activity of oolong tea ethanol extract (OTEE) and epigallocatechin gallate (EGCG) on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7). Methods A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EGCG toward RAW 264.7 cells. Interleukins (IL-6, IL-1β), tumor necrosis factor-α (TNF-α), and cyclooxigenase-2 (COX-2) levels were measured by ELISA, and nitric oxide (NO) levels measured by a nitrate/nitrite colorimetric assay to determine the inhibition activity of OTEE and EGCG. Results Lipopolysaccharide induction increases NO, COX-2, IL-6, IL-1β, and TNF-α levels compared with the untreated cell (negative control). The positive control, lipopolysaccharide-induced RAW 264.7 without treatments showed the highest level of all pro-inflammatory cytokines and modulators tested in this study. The positive control was used as standard to obtain OTEE and EGCG inhibition activity toward NO, COX-2, IL-6, IL-1β, and TNF-α. OTEE had a higher inhibition activity toward NO, COX-2, IL-6, and IL-1β than EGCG; the reverse was seen for TNF-α. However, both OTEE and EGCG suppressed production of NO, COX-2, IL-6, IL-1β, and TNF-α. Conclusions OTEE and EGCG have the potential for use as anti-inflammatory drugs, which is shown by their ability to reduce the production of NO, COX-2, IL-6, IL-1β, and TNF-α in active macrophages.