Sulforaphene in Raphanus sativus L. var. caudatus Alef increased in late-bolting stage as well as anticancer activity
10.1016/j.apjtb.2017.09.022
- Author:
Piman POCASAP
1
;
Natthida WEERAPREEYAKUL
2
;
Waraporn TANTHANUCH
3
;
Kanjana THUMANU
3
Author Information
1. Graduate School, Faculty of Pharmaceutical Sciences, Khon Kaen University
2. Faculty of Pharmaceutical Sciences, Khon Kaen University
3. Synchrotron Light Research Institute (Public Organization)
- Publication Type:Journal Article
- Keywords:
Anticancer activity;
Isothiocyanates;
Late-bolting stage;
Sulforaphane;
Sulforaphene;
Thai rat-tailed radish
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(11):998-1004
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To evaluate the concentration differences of sulforaphene and sulforaphane at various ages and in different parts of Raphanus sativus L. var. caudatus with respect to their potential cancer preventive effect on HCT116 colon cancer cells. Methods FTIR–ATR and GC–MS were used to characterize the isothiocyanates in the plant extracts followed by HPLC for quantification. Antiproliferation and apoptosis induction were determined by using MTT assay and flow cytometry, respectively. Results The respective rank of anticancer activity of Raphanus sativus were as follows: vegetative (3 week) < older rosette (4 week) < early-bolting (5 week) < senescence (7 week) < late-bolting (6 week). The low to high concentration of sulforaphene and sulforaphane occurred in the same stage order. Conclusions The reproductive parts (flower, pod, and dry seed) of Raphanus sativus have the greatest isothiocyanate concentration, evidenced by a sulforaphene concentration higher than the sulforaphane. This result should inform the selection of the most appropriate harvesting stage and plant part for use as a potential chemopreventive agent.
