Oxidative stress mitigation, kinetics of carbohydrate-enzymes inhibition and cytotoxic effects of flavonoids-rich leaf extract of Gazania krebsiana (Less.): An in vitro evaluation
- Author:
Fatai BALOGUN
1
;
Anofi Omotayo ASHAFA
1
Author Information
- Publication Type:Journal Article
- Keywords: Cytotoxicity; Flavonoids; Kinetics; Lethality; Radical scavenging
- From:Asian Pacific Journal of Tropical Biomedicine 2018;7(1):52-58
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the free radical scavenging, antidiabetic, kinetics of enzyme inhibition and cytotoxic potentials of flavonoid-rich extract of Gazania krebsiana leaves using standard methods. Methods: Antioxidant activity of the flavonoids was investigated at scavenging free radicals such as 1,1-diphenyl-2-picryl hydrazyl, nitric oxide, hydroxyl radical, reducing capabilities, 2,2-azinobis (3-ethylbenzothiazoline-6) sulphonic acid as well as metal chelating capability at different concentrations (125-1 000 μg/mL) while the antidiabetic activity was evaluated via the inhibition and kinetics of carbohydrate digestive enzymes including α-amylase, α-glucosidase, sucrase and maltase. Brine shrimp lethality assay was also employed to examine the cytotoxic effects at various concentrations (125-2 000 μg/mL). Results: The study showed the best antioxidant activity in 2,2-azinobis (3-ethylbenzothiazoline-6) sulphonic acid, hydroxyl radical, nitric oxide and metal chelating with IC
