Phytochemical investigation, antioxidant and wound healing activities of Citrullus colocynthis (bitter apple)
- Author:
Sateesh Chandra GUPTA
1
;
Tusha TRIPATHI
1
;
Annie G. AGARWAL
1
;
Om P. SIDHU
1
;
Shravan Kumar PASWAN
2
;
Chandana V. RAO
2
Author Information
- Publication Type:Journal Article
- Keywords: Antioxidant; Citrullus colocynthis; GC-MS; Metabolite profiling; Wound healing
- From:Asian Pacific Journal of Tropical Biomedicine 2018;8(8):418-424
- CountryChina
- Language:Chinese
- Abstract: Objective: To undertake metabolite profiling of various plant parts of Citrullus colocynthis, and assess antioxidant and wound healing activities of fractions for therapeutical applications. Methods: Extracts from leaves, stem, root, fruit pulp and seeds were analyzed using gas chromatography-mass spectrometry and high performance liquid chromatography. Variation in antioxidant potential was assayed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. The extract with highest antioxidant potential was subjected on in-vivo wound healing activity using excision wound model. Results: Metabolite profiling of Citrullus colocynthis identified 70 chemically diverse metabolites from different plant parts by using a combination of GC-MS and HPLC. Concentration of colocynthin, a principal active secondary metabolite, ranged from 3.15 mg/g dry weight to 242.00 mg/g dry weight, the lowest being in leaves and highest in fruit pulp. DPPH radical scavenging activity of free radical (IC
