Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells

Sivapragasam Gothai; Katyakyini Muniandy; Palanisamy Arulselvan; Norhaizan Mohd Esa; Suresh Kumar Subbiah; Palanisamy Arulselvan; Palanisamy Arulselvan

Return

Anticancer potential of Alternanthera sessilis extract on HT-29 human colon cancer cells

Author: Sivapragasam GOTHAI ¹ ; Katyakyini MUNIANDY ¹ ; Palanisamy ARULSELVAN ¹ ; Norhaizan Mohd ESA ² ; Suresh Kumar SUBBIAH ³ ; Palanisamy ARULSELVAN ⁴ ; Palanisamy ARULSELVAN ⁵
Author Information

1. Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia
2. Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
3. Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
4. Muthayammal Centre for Advanced Research, Muthayammal College of Arts and Science, Rasipuram
5. Scigen Research and Innovation Pvt. Ltd., Periyar Technology Business Incubator, Periyar Nagar
Publication Type:Journal Article
Keywords: Alternanthera sessilis; Anticancer; Apoptosis; Colorectal cancer; HT-29
From:Asian Pacific Journal of Tropical Biomedicine 2018;8(8):394-402
CountryChina
Language:Chinese
Abstract: Objective: To identify the bioactive extracts from Alternanthera sessilis and investigate its cytotoxicity potential against colon cancer cells, HT-29. Methods: This study examined the effects of three parts (aerial, leaf, stem) of whole plant on HT-29 colon cancer cell lines. Three different extracts from the plant parts were prepared by maceration technique using 80% ethanol. The anticancer activities were determined using MTT, clonogenic, cell motility and AOPI assay. The chemical composition profiling was analyzed by GC-MS. Results: Among three plant part extracts, leaf extract greatly suppressed the growth of colon cancer cells in time and dosage-dependent manner, followed by aerial and stem. The cytotoxicity results were rationalized with clonogenic, cell motility and AO/PI assay, where extract showed the most active activity compared to aerial and stem extracts. GC-MS analysis of leaf extract showed there were various recognized anti-cancer, anti-oxidant and anti-inflammatory compounds. Conclusions: Amid the screened extracts, the leaf extract exhibits the credible cytotoxic, anti-proliferative and apoptotic activity and hence, our findings call for additional research to conclude the active compounds and their mechanisms determining the apoptotic activity.