Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack

Dao Thi Thanh Hien; Tran Phi Long; Tran Phuong Thao; Jeong-hyung Lee; Duong Thu Trang; Nguyen Thi Thu Minh; Pham Van Cuong; Nguyen Hai Dang; Do Thi Ngoc Lan; Nguyen Tien Dat

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Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack

Author: Dao Thi Thanh HIEN ¹ ; Tran Phi LONG ² ; Tran Phuong THAO ² ; Jeong-Hyung LEE ² ; Duong Thu TRANG ³ ; Nguyen Thi Thu MINH ³ ; Pham VAN CUONG ³ ; Nguyen Hai DANG ³ ; Do Thi Ngoc LAN ⁴ ; Nguyen Tien DAT ⁵
Author Information

1. Deparment of Traditional Pharmacy, Hanoi University of Pharmacy
2. Department of Biochemistry, College of Natural Sciences, Kangwon National University
3. Advanced Center for Bio-organic Chemistry, Institute of Marine Biochemistry, Vietnam Academy of Science and Technology
4. National Institute of Drug Quality Control
5. Center for Research and Technology Transfers, VAST
Publication Type:Journal Article
Keywords: 10-dimethoxycanthin-6-one; Alkaloid,9; Anti-inflammation; Eurycoma longifolia
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(1):18-23
CountryChina
Language:Chinese
Abstract: Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The level of NO produced in the culture media was determined by Griess method. The iNOS and COX-2 protein expressions were analyzed by Western blot. The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model. Mice mortality was monitored for 5 days after injection of LPS. The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques. Results: The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vivo models. The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions. In the septic shock model, ELA dose-dependently protected mice from LPS-induced mortality. Further study on the isolated components of ELA indicated that 9,10-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract. Conclusions: These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS, and COX-2 and protects mice from LPS-induced mortality in septic shock model.