Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract

Gothai Sivaprakasam; Katyakyini Muniandy; Palanisamy Arulselvan; Palanivel Ganesan; Shin-young Park; Duk-yeon Cho; Joon-so Kim; Palanivel Ganesan; Dong-kug Choi; Palanisamy Arulselvan; Palanisamy Arulselvan; Dong-kug Choi

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Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract

Author: Gothai SIVAPRAKASAM ¹ ; Katyakyini MUNIANDY ¹ ; Palanisamy ARULSELVAN ¹ ; Palanivel GANESAN ² ; Shin-Young PARK ² ; Duk-Yeon CHO ² ; Joon-So KIM ² ; Palanivel GANESAN ³ ; Dong-Kug CHOI ³ ; Palanisamy ARULSELVAN ⁴ ; Palanisamy ARULSELVAN ⁵ ; Dong-Kug CHOI ⁶
Author Information

1. Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia
2. Department of Biomedical Chemistry, College of Biomedical and Health Science, Konkuk University
3. Nanotechnology Research Center, Konkuk University
4. Scigen Research and Innovation Pvt. Ltd., Periyar Technology Business Incubator
5. Muthayammal Centre for Advanced Research, Muthayammal College of Arts and Science
6. Department of Biotechnology, College of Biomedical and Health Science, Konkuk University
Publication Type:Journal Article
Keywords: BV2 microglial cells; Moringa oleifera leaf extract; Neuro-inflammation; NF-κB signaling pathway; Pro-inflammatory cytokines
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(3):109-115
CountryChina
Language:Chinese
Abstract: Objective: To determine the anti-neuroinflammatory activity of Moringa oleifera leaf extract (MLE) under lipopolysaccharide stimulation of mouse murine microglia BV2 cells in vitro. Methods: The cytotoxicity effect of MLE was investigated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay. The inflammatory response of BV-2 cells were induced with lipopolysaccharide. The generation of nitric oxide levels was determined by using Griess assay and the level of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) was evaluated by ELISA kit. The expression of iNOS, COX-2 as well as IκB-α was carried out by immunoblot analysis. Results: MLE reduced the nitric oxide production in concentration-dependent manner, and maintained the viability of BV-2 microglial cells which indicated absence of toxicity. In addition, MLE repressed the activation of nuclear factor kappa B by arresting the deterioration of IκB-α, consequently resulted in suppression of cytokines expression such as COX-2 and iNOS. Conclusions: MLE inhibitory activities are associated with the inhibition of nuclear factor kappa B transcriptional activity in BV2 microglial cells. Thus MLE may offer a substantial treatment for neuroinflammatory diseases.