Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats

Nurul Che Hamzah; Arifullah Mohammed; Mohd Asari; Zulhazman Hamzah; Ibrahim Shaik

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Keladi candik (Alocasia longiloba Miq.) petiole extracts promote wound healing in a full thickness excision wound model in rats

Author: Nurul CHE HAMZAH ¹ ; Arifullah MOHAMMED ¹ ; Mohd ASARI ² ; Zulhazman HAMZAH ³ ; Ibrahim SHAIK ⁴
Author Information

1. Faculty of Agro-Based Industry, Universiti Malaysia Kelantan
2. Department of Anatomy, School of Medical Sciences, Universiti Sains Malaysia, Health Campus
3. Faculty of Sains Bumi, Universiti Malaysia Kelantan
4. Faculty of Pharmaceutical Sciences, UCSI University
Collective Name:Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia
Publication Type:Journal Article
Keywords: Alocasia longiloba petiole extract; Full thickness excision wound; Rat model; Sprague-dawley rat
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(4):140-149
CountryChina
Language:Chinese
Abstract: Objective: To investigate the wound-healing effect of Alocasia longiloba (A. longiloba) petiole extract on wounds in rats. Methods: Twenty-two male Sprague-dawley rats were randomly assigned to receive 10% solcoseryl gel, phosphate buffer saline, 50% ethanol, 95% ethanol and hexane extracts of A. longiloba at 1.5%, 3% and 6% doses, respectively. A full thicknesses wound (6 mm) was created on the dorsal of the rat; and all rats were applied with the extract solutions, 10% solcoseryl gel and phosphate buffer saline once a day topically until day 12. The wound was photographed on day 1, 6 and 12, and the percentage of wound contraction was calculated. On day 12, rats were sacrificed and histological examination of granulation tissue was carried out using haematoxylin & eosin and Masson's Trichrome stain to determine the wound healing effect. Results: In this study, 6% of 50% and 95% ethanol extracts of A. longiloba showed 82.50% and 82.32% wound contraction, respectively, and were comparable with 10% solcoseryl gel (82.30%). Meanwhile, phosphate buffer saline treated group showed the lowest wound contraction (69.86%). Histological assessment of wound treated with 6% of 95% ethanol extract of A. longiloba showed distinct epidermal and dermal layer, higher proliferation of fibroblast and more angiogenesis with collagen compared to other wound treated groups. Conclusions: A. longiloba petiole extracts have a wound healing potential and 6% of 95% ethanol extract of A. longiloba is more effective. Further studies are required to understand the wound healing mechanism of action of the extract.