Methanolic extract of Abrus precatorius promotes breast cancer MDA-MB-231 cell death by inducing cell cycle arrest at G

Author: Wan WAN-IBRAHIM ¹ ; Norzila ISMAIL ¹ ; Siti MOHD-SALLEH ¹ ; Aidy YAJID ² ; Michael WONG ³ ; Mohd MD HASHIM ³
Author Information

1. Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia
2. Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia
3. Department of Surgery, School of Medical Sciences, Universiti Sains Malaysia
Publication Type:Journal Article
Keywords: Abrus precatorius; Apoptosis; Breast cancer; Cell cycle; MDA-MB-231
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(6):249-256
CountryChina
Language:Chinese
Abstract: Objective: To determine the anti-proliferative activity of Abrus precatorius (A. precatorius) leaf extracts and their effect on cell death. Methods: A. precatorius leaves were extracted successively with hexane, ethyl acetate and methanol by Soxhlet extraction. Aqueous extract was prepared by decoction at 50 C. Extracts of A. precatorius leaves were used to treat selected cancer and normal cell lines for 72 h. Furthermore, 3-(4,5-dimethyl thiazol-2-yl) 2,5-diphenyl tetrazolium bromide assay was performed to determine cell viability. Analysis of cell cycle arrest, apoptosis assay and apoptosis protein expressions were determined by flow cytometry. Results: Methanolic extract of A. precatorius leaves showed the lowest IC