Cytotoxic, apoptotic and cell migration inhibitory effects of atranorin on SPC212 mesothelioma cells

Author: Erhan SAHIN ¹ ; Varol SAHINTURK ¹ ; Sinem PSAV ² ; Mehmet CANDAN ² ; Ayse KOPARAL ² ; Ilker AVAN ³
Author Information

1. Histology and Embryology Department, Medicine School, Eskisehir Osmangazi University
2. Biology Department, Faculty of Science, Eskisehir Technical University
3. Chemistry Department, Faculty of Science, Eskisehir Technical University
Publication Type:Journal Article
Keywords: Atranorin; Malignant mesothelioma; SPC212 cell line
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(7):299-306
CountryChina
Language:Chinese
Abstract: Objective: To investigate the effects of atranorin, a lichen secondary metabolite, on SPC212 malignant mesothelioma cells in vitro. Methods: SPC212 malignant mesothelioma cell line was used. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to evaluate cytotoxic effects of atranorin and cisplatin at 24, 48 and 72 h. Hematoxylin-eosin staining and 4',6-diamidino-2-phenylindole, dihydrochloride staining were used for determining cell and nucleus morphology, respectively. Wound healing assay was used for investigating cell migration. The xCELLigence real-time cell analysis system was used for determining cell proliferation. Results: Atranorin at 5-450 μΜ decreased cell viability at 24, 48 and 72 h. IC