Cytotoxic, apoptotic and cell migration inhibitory effects of atranorin on SPC212 mesothelioma cells
- Author:
Erhan SAHIN
1
;
Varol SAHINTURK
1
;
Sinem PSAV
2
;
Mehmet CANDAN
2
;
Ayse KOPARAL
2
;
Ilker AVAN
3
Author Information
- Publication Type:Journal Article
- Keywords: Atranorin; Malignant mesothelioma; SPC212 cell line
- From:Asian Pacific Journal of Tropical Biomedicine 2019;9(7):299-306
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the effects of atranorin, a lichen secondary metabolite, on SPC212 malignant mesothelioma cells in vitro. Methods: SPC212 malignant mesothelioma cell line was used. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to evaluate cytotoxic effects of atranorin and cisplatin at 24, 48 and 72 h. Hematoxylin-eosin staining and 4',6-diamidino-2-phenylindole, dihydrochloride staining were used for determining cell and nucleus morphology, respectively. Wound healing assay was used for investigating cell migration. The xCELLigence real-time cell analysis system was used for determining cell proliferation. Results: Atranorin at 5-450 μΜ decreased cell viability at 24, 48 and 72 h. IC