Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1

Mohamed Abdikarim; Muhammad Abbas; Munirah Zakaria; Rapeah Suppian; Muhammad Abbas; Robaiza Zakaria

Return

Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1

Author: Mohamed ABDIKARIM ¹ ; Muhammad ABBAS ¹ ; Munirah ZAKARIA ¹ ; Rapeah SUPPIAN ¹ ; Muhammad ABBAS ² ; Robaiza ZAKARIA ³
Author Information

1. Biomedicine Programme, School of Health Sciences, Health Campus, Universiti Sains Malaysia
2. Department of Human Physiology, Faculty of Basic Medical Sciences, Bayero University Kano
3. Department of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia
Publication Type:Journal Article
Keywords: Adjuvant rBCG; Immune; MSP-1; TLR-2
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(7):271-277
CountryChina
Language:Chinese
Abstract: Objective: To determine the effects of toll-like receptor 2 (TLR-2) agonist, Pam3CSK4, on cellular and humoral immune response against recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum. Methods: Six groups of mice (n=6 per group) received intraperitoneal phosphate buffered saline T80 (PBS-T80), BCG or rBCG in the presence or absence of Pam3CSK4. Enzyme-linked immunosorbent assay was carried out to measure serum total IgG, IgG1, IgG2a, and IgG2b production. Spleens were also harvested and splenocytes were co-cultured with rBCG antigen for in vitro determination of IL-4 and IFN-γ via enzyme-linked immunosorbent assay. Results: The production of total IgG and the isotype IgG1, IgG2a and IgG2b was significantly higher in rBCG-immunised mice than in the BCG and PBS-T80-immunised mice, and Pam3CSK4 further enhanced their productions. A similar pattern was also observed in IFN-γ production. Moreover, there was no significant difference in IL-4 production in all groups either in the presence or absence of Pam3CSK4. Conclusions: We present evidence of the adjuvant effects of TLR-2 agonist in enhancing the production of total IgG, IgG1, IgG2a, IgG2b, as well as IFN-γ in response to rBCG. However, the presence or absence of Pam3CSK4 had no effect on IL-4 production.