Cytotoxic effect of methanolic extracts of Fritillaria imperialis bulbs and Eryngium caucasicum leaves on hepatoma and colon cancer cells

Mostafa Kardan; Zahra Yazdani; Zaher Morsaljahan; Alireza Rafiei; Mohammad Ebrahimzadeh

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Cytotoxic effect of methanolic extracts of Fritillaria imperialis bulbs and Eryngium caucasicum leaves on hepatoma and colon cancer cells

Author: Mostafa KARDAN ¹ ; Zahra YAZDANI ² ; Zaher MORSALJAHAN ² ; Alireza RAFIEI ² ; Mohammad EBRAHIMZADEH ³
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1. Department of Immunology, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences
2. Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences
3. Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Mazandaran University of Medical Sciences
Publication Type:Journal Article
Keywords: Colon cancer; Cytotoxity; Eryngium caucasicum; Fritillaria imperialis; Hepatoma cancer
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(8):353-358
CountryChina
Language:Chinese
Abstract: Objective: To evaluate antitumor activities of Fritillaria imperialis and Eryngium caucasicum methanolic extracts on human hepatoma (HepG2) and colon cancer (HCT116) cell lines in comparison to human foreskin fibroblasts as the normal cells. Methods: Methanolic extracts of Fritillaria imperialis and Eryngium caucasicum were prepared by the maceration method. The effect of the extracts at various concentrations (100, 200, 400, 600, and 800 μg/mL) on cell survival was evaluated using the MTT method. Besides, fluorescence staining was used to evaluate death patterns of the cells. Results: MTT assay showed that Fritillaria imperialis significantly decreased the viability of all cell lines after 24 and 48 hours of treatments. However, Eryngium caucasicum extract did not show any significant cytotoxicity effect on the cell lines. Fluorescence staining revealed that Fritillaria imperialis induced apoptosis of HCT116 cells at 550 μg/mL. Conclusions: Fritillaria imperialis extract has antiproliferative and cytotoxic effects on HCT116 and HepG2 cancer cells and therefore, may serve as an anticancer agent.