Prediction of T cell and B cell epitopes of the 22-, 47-, 56-, and 58-kDa proteins of Orientia tsutsugamushi

Li-na Niu; Ting-ting FU; Yu-ying Dong; Zi-hao Wang; Si-qi Wang; Xuan Zhao; Nai-xu Hou; Qiang WU; Qian Chen; Man-ling Chen; Jin-chun TU; Li-na Niu; Qiang WU

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Prediction of T cell and B cell epitopes of the 22-, 47-, 56-, and 58-kDa proteins of Orientia tsutsugamushi

Author: Li-Na NIU ¹ ; Ting-Ting FU ¹ ; Yu-Ying DONG ¹ ; Zi-Hao WANG ¹ ; Si-Qi WANG ¹ ; Xuan ZHAO ¹ ; Nai-Xu HOU ¹ ; Qiang WU ¹ ; Qian CHEN ² ; Man-Ling CHEN ³ ; Jin-Chun TU ⁴ ; Li-Na NIU ⁵ ; Qiang WU ⁶
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1. Laboratory of Tropical Biomedicine and Biotechnology, School of Tropical Medicine and Laboratory Medicine, Hainan Medical University
2. Department of Laboratory, First Affiliated Hospital of Hainan Medical University
3. Department of Gynecology, First Affiliated Hospital of Hainan Medical University
4. State Key Laboratory of Marine Resource Utilization in South China Sea, College of Material Science and Engineering, Hainan University
5. Department of Pathogen Biology, School of Basic Medicine and Life Science, Hainan Medical University, Hainan Medical University-University of Hong Kong Joint Laboratory of Tropical Infectious Diseases
6. Key Laboratory of Emergency and Trauma, Ministry of Education, Research Unit of Island Emergency Medicine, Chinese Academy of Medical Sciences, Hainan Medical University
Publication Type:Journal Article
Keywords: B cell epitopes; Bioinformatic prediction; Orientia tsutsugamushi; T cell epitopes
From:Asian Pacific Journal of Tropical Biomedicine 2019;9(10):443-448
CountryChina
Language:Chinese
Abstract: Objective: To predict B cell and T cell epitopes of 22-kDa, 47-kDa, 56-kDa and 58-kDa proteins. Methods: The sequences of 22-kDa, 47-kDa, 56-kDa and 58-kDa proteins which were derived from Orientia tsutsugamushi were analyzed by SOPMA, DNAstar, Bcepred, ABCpred, NetMHC, NetMHC II and IEDB. The 58-kDa tertiary structure model was built by MODELLER9.17. Results: The 22-kDa B-cell epitopes were located at positions 194-200, 20-26 and 143-154, whereas the T-cell epitopes were located at positions 154-174, 95-107, 17-25 and 57-65. The 47-kDa protein B-cell epitopes were at positions 413-434, 150-161 and 283-322, whereas the T-cell epitopes were located at positions 129-147, 259-267, 412-420 and 80-88. The 56-kDa protein B-cell epitopes were at positions 167-173, 410-419 and 101-108, whereas the T-cell epitopes were located at positions 88-104, 429-439, 232-240 and 194-202. The 58-kDa protein B-cell epitopes were at positions 312-317, 540-548 and 35-55, whereas the T-cell epitopes were located at positions 415-434, 66-84 and 214-230. Conclusions: We identified candidate epitopes of 22-kDa, 47-kDa, 56-kDa and 58-kDa proteins from Orientia tsutsugamushi. In the case of 58-kDa, the dominant antigen is displayed on tertiary structure by homology modeling. Our findings will help target additional recombinant antigens with strong specificity, high sensitivity, and stable expression and will aid in their isolation and purification.