Raspberry ketone attenuates high-fat diet-induced obesity by improving metabolic homeostasis in rats

Ali Alkaladi; Mohammed Baeshen; Haytham Ali; Mohamed Afifi; Ammar Al-farga; Haytham Ali; Aaser Abdelazim; Mohamed Afifi; Aaser Abdelazim

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Raspberry ketone attenuates high-fat diet-induced obesity by improving metabolic homeostasis in rats

Author: Ali ALKALADI ¹ ; Mohammed BAESHEN ¹ ; Haytham ALI ² ; Mohamed AFIFI ² ; Ammar AL-FARGA ² ; Haytham ALI ³ ; Aaser ABDELAZIM ³ ; Mohamed AFIFI ³ ; Aaser ABDELAZIM ⁴
Author Information

1. University of Jeddah, College of Science, Department of Biology
2. University of Jeddah, College of Science, Department of Biochemistry
3. Department of Biochemistry, Faculty of Vet. Medicine, Zagazig University
4. Department of Basic Medical Sciences, College of Applied Medical Sciences, University of Bisha
Publication Type:Journal Article
Keywords: Molecular mechanism; Obesity; Raspberry ketone
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(1):18-22
CountryChina
Language:Chinese
Abstract: To investigate the molecular mechanisms of the antiobese effect of raspberry ketone against high-fat diet fed rats. Methods: Fifty adult male rats were randomly assigned to receive a standard diet, a high fat diet, and the high-fat diet and 0.5%, 1% or 2% raspberry ketone. Body weight, biochemical parameters and gene expression of CCAAT enhancer-binding protein (C/EBP)-d, fatty acid synthase (FAS), acetyl CoA carboxylase (ACC), peroxisome proliferator-activated receptor alpha (PPAR-a), hormone-sensitive lipase (HSL) and hepatic carnitine palmitoyltransferase 1 A (CPT1A) were investigated. Results: Body weight, blood glucose, insulin, total lipids, triacylglycerols, total cholesterol and low-density lipoprotein cholesterol were increased in high-fat diet fed rats. These high fat diet-induced changes were attenuated by treatment with raspberry ketone. High-density lipoprotein cholesterol was decreased in highfat diet fed rats but increased in rats treated with raspberry ketone. Molecular investigations showed induction of gene expression of C/EBP-d , FAS, ACC, CPT1A and inhibition of gene expression of PPAR-a and HSL in high-fat diet fed rats as compared with control. Raspberry ketone treament reversed these changes except CPT1A. Conclusions: Raspberry ketone can prevent obesity induced by a high-fat diet in rats by induction of the expression of enzymes, controlling lipolysis and fatty acids b oxidation as well as inhibition of gene expressions of adipogenic factors.