Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities

Shaymaa Fadhel Abbas Albaayit; Abdullah Rasedee; Shaymaa Fadhel Abbas Albaayit; Noorlidah Abdullah; Yusuf Abba

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Methanolic extract of Clausena excavata promotes wound healing via antiinflammatory and anti-apoptotic activities

Author: Shaymaa Fadhel Abbas ALBAAYIT ¹ ; Abdullah RASEDEE ² ; Shaymaa Fadhel Abbas ALBAAYIT ³ ; Noorlidah ABDULLAH ³ ; Yusuf ABBA ⁴
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1. Department of Biology, College of Science, University of Baghdad
2. Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia
3. Institute of Biological Sciences, Faculty of Science, University of Malaya
4. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia
Publication Type:Journal Article
Keywords: Anti-inflammatory cytokines; Apoptotic genes; Clausena excavata leaves; Cytotoxicity
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(5):232-238
CountryChina
Language:Chinese
Abstract: Objective: To investigate the anti-inflammatory properties of methanolic extract of Clausena excavata in lipopolysaccharide (LPS)-activated macrophages (J774A.1) and the effect on skin wound in a rat model through determining cytokine levels and gene expressions. Methods: The effects of methanolic extract of Clausena excavata on in vitro viability and TNF-α, IL-6, IL-10, and nitric oxide release by LPS-activated J774A.1 cells were determined. In addition, relative expressions of BAX, BCL-2 and COX-2 genes were examined in healed wounds of rats. Results: The methanolic extract of Clausena excavata was not toxic to J774A.1 cells at the highest dose of 400 μg/mL. It decreased levels of TNF-α and IL-6, while increasing IL-10 level in LPS-activated J774A.1 cells and in the healed wounds of rats. The methanolic extract of Clausena excavata also inhibited nitric oxide production in LPS-activated J774A.1 cells. The BAX and COX-2 genes were downregulated while the BCL-2 gene was upregulated in the healed wound of rats. Conclusions: The methanolic extract of Clausena excavata promotes wound healing via its anti-inflammatory and anti-apoptotic activities.