Crebanine N-oxide, a natural aporphine alkaloid isolated from Stephania hainanensis, induces apoptosis and autophagy in human gastric cancer SGC-7901 cells

Author: Zheng-Wen WANG ¹ ; Hao LIU ¹ ; Geng-Tai YE ¹ ; Zhi-Yong SHENG ¹ ; Yan-Feng HU ¹ ; Guo-Xin LI ¹ ; Zheng-Wen WANG ² ; Yin-Feng TAN ³
Author Information

1. Department of General Surgery, Nanfang Hospital, Southern Medical University
2. Department of Hepatopancreatobiliary Surgery and General Surgery, Hainan Cancer Hospital, Affiliated Cancer Hospital of Hainan Medical University
3. Hainan Provincial Key Laboratory of R and D on Tropical Herbs, Hainan Medical University
Publication Type:Journal Article
Keywords: Apoptosis; Autophagy; Crebanine N-oxide; Gastric cancer; SGC-7901 cells
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(5):224-231
CountryChina
Language:Chinese
Abstract: Objective: To investigate the cytotoxic effects and the potential mechanisms of crebanine N-oxide in SGC-7901 gastric adenocarcinoma cells. Methods: The cytotoxicity of crebanine N-oxide was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay and cellular morphology was observed under a microscope. Cell apoptosis was determined by flow cytometry using propidium iodide staining. The expression levels of apoptotic-related proteins, cleaved caspase-3, cytochrome C, p53 and Bax, and autophagy-related proteins p62, beclin1 and LC3 were detected by Western blotting assays. Results: Crebanine N-oxide treatment significantly inhibited the proliferation of SGC-7901 cells in a dose-dependent and time-dependent manner via induction of G