Response surface methodology-based optimization of ultrasound-assisted extraction of β-sitosterol and lupeol from astragalus atropilosus (roots) and validation by HPTLC method

Author: Perwez ALAM ¹ ; Nasir A. SIDDIQUI ¹ ; Ali S. ALQAHTANI ¹ ; Omer A. BASUDAN ¹ ; Saleh I. ALQASOUMI ¹ ; Abdullah A. AL-MISHARI ¹ ; Anzarul HAQUE ²
Author Information

1. Department of Pharmacognosy, College of Pharmacy, King Saud University
2. Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University
Collective Name:Department of Pharmaceutical Chemistry and Pharmacognosy, Unaizah College of Pharmacy, Qassim University
Publication Type:Journal Article
Keywords: Astragalus; Box-Behnken design; High performance thin layer chromatography; Lupeol; β-sitosterol
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(6):281-292
CountryChina
Language:Chinese
Abstract: Objective: To optimize the ultrasonication method for efficient extraction of P-sitosterol and lupeol from the roots of Astragalus atropilosus using Box-Behnken design of response surface methodology (RSM), and its validation by high performance thin layer chromatography (HPTLC) method. Methods: Ultrasonication method was used to extract β-sitosterol and lupeol from Astragalus atropilosus (roots). RSM was used to optimize the different extraction parameters viz. liquid to solid ratio (10-14 mL/g), temperature (60-80 °C) and time (40-60 min) to maximize the yield of β-sitosterol and lupeol. The quantitative estimation of β-sitosterol and lupeol was done in chloroform extract of Astragalus atropilosus by validated HPTLC method on 10 cm × 20 cm glass-backed silica gel 60F