Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice

Byoung Cho; Jae Shin; Hyun Kang; Seon Jang; Byoung Cho; Ji-su Kim; Denis Che; Seon Jang; Ji-su Kim; Denis Che; Hyeon Kang; Hyeonhwa OH; Young-soo Kim

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Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice

Author: Byoung CHO ¹ ; Jae SHIN ¹ ; Hyun KANG ¹ ; Seon JANG ¹ ; Byoung CHO ² ; Ji-Su KIM ² ; Denis CHE ² ; Seon JANG ² ; Ji-Su KIM ^{3
,

4} ; Denis CHE ⁵ ; Hyeon KANG ⁵ ; Hyeonhwa OH ⁵ ; Young-Soo KIM ⁵
Author Information

1. Research Institute, Ato QandA Co. Ltd
2. Department of Health Management, Jeonju University
3. Department of Health Management
4. Agro-Bio and Food Industry, Jeonju University
5. Department of Food Science and Technology, Chonbuk National University
Publication Type:Journal Article
Keywords: Atopic dermatitis; Cytokine; Date plum leaves; Enzyme; Inflammation
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(6):239-247
CountryChina
Language:Chinese
Abstract: Objective: To evaluate the effect of different extracts of Diospyros lotus leaves in atopic dermatitis Methods: Diospyros lotus leaves were extracted in ethanol and treated with or without hydrochloric acid or α-rhamnosidase to obtain three different extracts-ethanol, acid-hydrolyzed, and enzyme-hydrolyzed leaf extracts of date plum. The myricitrin content in all samples was measured using HPLC analysis. In vitro antioxidant and anti-inflammatory activities of the extracts were determined by measuring DPPH radical scavenging activities and nitric oxide production in RAW264.7 cells, respectively. Seven-week-old male hairless mice were used to evaluate the anti-atopic dermatitis effects of three extracts in vivo. Splenocytes and mast cells were used to further determine the anti-atopic dermatitis effects of the major compound in the ethanol leaf extract. Results: Enzyme-hydrolyzed leaf extract showed significant in vitro antioxidant and anti-inflammatory activities, and attenuated atopic dermatitis-like skin symptoms and clinical signs more significantly than ethanol and acid-hydrolyzed leaf extracts in 1-fluoro-2,4-dinitrobenzene and house dust mite antigen-treated hairless mice. Enzyme-hydrolyzed leaf extract also suppressed the serum level of immunoglobulin E, interleukin (IL)-4, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, thymic stromal lymphopoietin, and thymus and activation-regulated chemokine in mice with atopic dermatitis. Furthermore, histological analysis revealed that enzyme-hydrolyzed leaf extract suppressed the increased epidermal thickness, dermal infiltration of inflammatory cells, and infiltration and degranulation of mast cells more markedly than the other two extracts in atopic dermatitis-like skin lesions. In addition, this extract effectively inhibited the production of IFN-γ, IL-4,and thymus and activation-regulated chemokine compared with the other two extracts in concanavalin A-stimulated splenocytes. Myricitrin, a major compound of enzyme-hydrolyzed leaf extract, suppressed atopic dermatitis biomarkers in stimulated mouse splenocytes and HMC-1 human mast cells. Conclusions: These results suggest that enzyme-hydrolyzed leaf extract might be a potential candidate to treat atopic dermatitis.