Antibiofilm activity of alpha-mangostin loaded nanoparticles against Streptococcus mutans

Phuong Nguyen; Lien Quach; Dong Quyen; Phuong Nguyen; Minh Nguyen; Lam Nguyen; Dong Quyen

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Antibiofilm activity of alpha-mangostin loaded nanoparticles against Streptococcus mutans

Author: Phuong NGUYEN ¹ ; Lien QUACH ¹ ; Dong QUYEN ¹ ; Phuong NGUYEN ² ; Minh NGUYEN ³ ; Lam NGUYEN ³ ; Dong QUYEN ³
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1. Institute of Biotechnology, Vietnam Academy of Science and Technology
2. Institute of Graduate University of Science and Technology, Vietnam Academy of Science and Technology
3. University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology
Publication Type:Journal Article
Keywords: Alpha-mangostin; Antibiofilm activity; Nanoparticle; Streptococcus mutans
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(7):325-332
CountryChina
Language:Chinese
Abstract: Objective: To investigate the antibiofilm activity of alpha- mangostin (AMG) loaded nanoparticle (nanoAMG) against dental caries pathogen Streptococcus mutans. Methods: AMG was isolated from the peels of Garcinia mangostana L. using silica gel columns and chemically analysed by high performance liquid chromatography and nuclear magnetic resonance. NanoAMG was prepared using the solvent evaporation method combined with high-speed homogenization. The nanoparticles were characterized using dynamic light scattering, field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR). The toxicity of nanoAMG in fibroblast NIH/3T3 cell line was determined using MTT method. The antibiofilm effect of nanoAMG was determined through the evaluation of biofilm formation by Streptococcus mutans using a 96-well plate. Biofilm biomass was quantified using crystal violet. Cell viability was observed under confocal microscopy using LIVE/DEAD BacLight staining. Moreover, gene expression was determined by quantitative real-time PCR and membrane permeabilization activity by measuring the uptake of o-nitrophenol- β-D-galactoside. Results: NanoAMG size was in a range of 10-50 nm with a polydispersity index of < 0.3 and zeta potential value of -35.2 mV The size and the incorporation of AMG in the nanoparticles were confirmed by FE-SEM and FTIR analyses. The IC