In vitro antioxidant and anti-cancer activities and phytochemical analysis of Commelina benghalensis L. root extracts

Riffat Batool; Ejaz Aziz; Javed Iqbal; Tariq Mahmood; Hina Salahuddin; Benny Tan; Saira Tabassum

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In vitro antioxidant and anti-cancer activities and phytochemical analysis of Commelina benghalensis L. root extracts

Author: Riffat BATOOL ¹ ; Ejaz AZIZ ² ; Javed IQBAL ³ ; Tariq MAHMOOD ³ ; Hina SALAHUDDIN ⁴ ; Benny TAN ⁵ ; Saira TABASSUM ⁶
Author Information

1. University Institute of Biochemistry and Biotechnology, PMAS-UAAR
2. Department of Botany, Gdc Khanpur
3. Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University
4. Department of Zoology, University of Okara
5. Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore
6. Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University
Publication Type:Journal Article
Keywords: Anti-cancer; Antioxidant; Commelina benghalensis; FT-IR
From:Asian Pacific Journal of Tropical Biomedicine 2020;10(9):417-425
CountryChina
Language:Chinese
Abstract: Objective: To explore antioxidant potential, anti-cancer activity, and phytochemicals of Commelina benghalensis L. Methods: The roots of Commelina benghalensis were extracted in different solvents (methanol, ethanol, benzene, chloroform, n-hexane) with a range of polarity. Antioxidant activity was evaluated by reducing power assay, DPPH radical scavenging activity and phosphomolybdenum method, cytotoxicity by MTT assay, apoptotic and cell cycle analysis by flow cytometry, migratory and invasive potential by wound scratch assay and invasion assay, respectively, functional groups analysis by FT-IR spectroscopy and phytochemicals by aluminum chloride colorimetric and Folin-Ciocalteu methods. Results: The extracts showed worthy antioxidant potential. The chloroform extract demonstrated the most significant cytotoxic effect on MDA-MB-231 (breast cancer) cell line, induced apoptosis and reduced migratory and invasive potential of MDA-MB-231 cells. Methanol and ethanol extracts presented good yield of total phenolic and total flavonoid contents. The FTIR spectroscopic studies revealed different characteristic peak values with various functional compounds such as alkenes, alkanes, aliphatic amines, aromatics, alkyl halides, carboxylic acid, alcohols, ester, aldehydes and ketones. Conclusions: The results demonstrate the potential use of Commelina benghalensis as a good antioxidant with significant anti-cancer effect.