Sang-Yod rice bran hydrolysates alleviate hypertension, endothelial dysfunction, vascular remodeling, and oxidative stress in nitric oxide deficient hypertensive rats

Gulladawan JAN-ON; Akarachai TUBSAKUL; Weerapon SANGARTIT; Poungrat PAKDEECHOTE; Upa KUKONGVIRIYAPAN; Gulladawan JAN-ON; Veerapol KUKONGVIRIYAPAN; Ketmanee SENAPHAN; Chakree THONGRAUNG

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Sang-Yod rice bran hydrolysates alleviate hypertension, endothelial dysfunction, vascular remodeling, and oxidative stress in nitric oxide deficient hypertensive rats

Author: Gulladawan JAN-ON ¹ ; Akarachai TUBSAKUL ¹ ; Weerapon SANGARTIT ¹ ; Poungrat PAKDEECHOTE ¹ ; Upa KUKONGVIRIYAPAN ¹ ; Gulladawan JAN-ON ² ; Veerapol KUKONGVIRIYAPAN ³ ; Ketmanee SENAPHAN ⁴ ; Chakree THONGRAUNG ⁵
Author Information

1. Department of Physiology, Faculty of Medicine, Khon Kaen University
2. School of Medicine, Walailak University
3. Department of Pharmacology, Faculty of Medicine, Khon Kaen University
4. Division of Physiology, Faculty of Veterinary Medicine, Khon Kaen University
5. Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University
Publication Type:Journal Article
Keywords: Endothelial dysfunction; Hypertension; Oxidative stress; Sang-Yod rice bran hydrolysates; Vascular remodeling
From:Asian Pacific Journal of Tropical Biomedicine 2021;11(1):10-19
CountryChina
Language:Chinese
Abstract: Objective: To evaluate the potential therapeutic effect of Sang-Yod rice bran hydrolysates (SRH) and in combination with lisinopril against hypertension, endothelial dysfunction, vascular remodeling, and oxidative stress in rats with nitric oxide deficiency-induced hypertension. Methods: Hypertension was induced in male Sprague-Dawley rats by administration of a nitric oxide synthase inhibitor, Nω- nitro-L-arginine methyl ester (L-NAME) in drinking water for 6 weeks. Hypertensive rats were administered daily with SRH (500 mg/kg/day), lisinopril (1 mg/kg/day), or the combination of SRH and lisinopril by gastric lavage for the last 3 weeks of L-NAME treatment. Hemodynamic status, vascular reactivity to vasoactive agents, and vascular remodeling were assessed. Blood and aortic tissues were collected for measurements of oxidative stress markers, plasma angiotensin-converting enzyme (ACE) activity, plasma angiotensin II, and protein expression. Results: L-NAME induced remarkable hypertension and severe oxidative stress, and altered contents of smooth muscle cells, elastin, and collagen of the aortic wall. SRH or lisinopril alone reduced blood pressure, restored endothelial function, decreased plasma ACEs and angiotensin II levels, alleviated oxidant markers and glutathione redox status, and restored the vascular structure. The effects were associated with increased expression of endothelial nitric oxide synthase and decreased expression of gp91phox and AT1R expression. The combination of SRH and lisinopril was more effective than monotherapy. Conclusions: SRH alone or in combination with lisinopril exert an antihypertensive effect and improve endothelial function and vascular remodeling through reducing oxidative stress and suppressing elevated renin-angiotensin system.