Aloin attenuates chronic constriction injury-induced neuropathic pain in rats by inhibiting inflammatory cytokines and oxidative stress

Aarti Kale; Avinash Wadkar; Umesh Mahajan; Lalit Birari; Chandragouda Patil; Kalpesh Patil; Sateesh Belemkar; Sameer Goyal; Shreesh Ojha; Sanjay Surana

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Aloin attenuates chronic constriction injury-induced neuropathic pain in rats by inhibiting inflammatory cytokines and oxidative stress

Author: Aarti KALE ¹ ; Avinash WADKAR ¹ ; Umesh MAHAJAN ¹ ; Lalit BIRARI ¹ ; Chandragouda PATIL ¹ ; Kalpesh PATIL ¹ ; Sateesh BELEMKAR ² ; Sameer GOYAL ³ ; Shreesh OJHA ⁴ ; Sanjay SURANA ⁵
Author Information

1. Department of Pharmacology, R. C. Patel Institute of Pharmaceutical Education and Research
2. School of Pharmacy and Technology Management, SVKM's Nmims, Mptp
3. Shri Vile Parle Kelavani Mandal's Institute of Pharmacy
4. Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, United Arab Emirates University
5. Department of Pharmacognosy, R. C. Patel Institute of Pharmaceutical Education and Research
Publication Type:Journal Article
Keywords: Aloin; Antioxidant; Chronic constriction injury; Neuropathic pain
From:Asian Pacific Journal of Tropical Biomedicine 2021;11(5):205-213
CountryChina
Language:Chinese
Abstract: Objective: To investigate the effect of aloin against chronic constriction injury (CCI)-induced neuropathic pain in rats. Methods: Rats were randomly divided into 7 groups: Group I (normal control), Group II (sham-operated), Group III (CCI control) and Group IV, V, VI, and VII, which underwent CCI surgery and then were administered with aloin (5 mg/kg, p.o.; 25 mg/kg, p.o.; 125 mg/kg, p.o.) and gabapentin (50 mg/kg, p.o.), respectively for 14 days. Peripheral neuropathy was induced by silk ligatures (4-0) loosely placed around the sciatic nerve. Nociceptive thresholds against mechanical stimuli (Von-Frey filaments) and thermal stimuli (12 °C and 40 °C) were measured at mid-plantar paw region ipsilateral to the compressed nerve on day-3, 7, 11, and 14. The concentration of cytokines including tumor necrosis factor-α (TNF-α), interleukin-6, and interleukin-1β was estimated at day-7. At day 14, motor nerve conduction velocity was determined under urethane anesthesia (1.25 g/kg). Oxidative stress parameters (malondiadehyde, glutathione, catalase, and superoxide dismutase) were estimated in sciatic nerve homogenates at day 14. Representative nerve samples were processed for histological investigations. Results: Aloin significantly reduced CCI-induced mechanical and thermal allodynia. It also improved motor nerve conduction velocity and decreased oxidative stress in nerve tissues. In addition, it decreased pro-inflammatory cytokine levels and restored the histoarchitecture of compressed sciatic nerve. Conclusions: Aloin mitigates CCI-induced neuropathic pain in rats by inhibiting oxidative stress and pro-inflammatory cytokines in the afflicted sciatic nerve.