Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65, c-Jun, and IRF3 signaling pathways

Li-shan Yan; Li Wang; Yu Ding; Jing Kong; Shuo-feng Zhang; Gan Luo; Yi Zhang; Brian Cheng; Qing Wang; Xiu-qiong FU

Return

Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65, c-Jun, and IRF3 signaling pathways

Author: Li-Shan YAN ¹ ; Li WANG ¹ ; Yu DING ¹ ; Jing KONG ¹ ; Shuo-Feng ZHANG ¹ ; Gan LUO ¹ ; Yi ZHANG ¹ ; Brian CHENG ² ; Qing WANG ³ ; Xiu-Qiong FU ⁴
Author Information

1. School of Chinese Materia Medica, Beijing University of Chinese Medicine
2. College of Professional and Continuing Education, Hong Kong Polytechnic University
3. The First Affiliated Hospital, Guangxi University of Chinese Medicine
4. Centre for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University
Publication Type:Journal Article
Keywords: Cytokines; Flavonoids; Macrophages; Proinflammatory; Saussurea involucrate
From:Asian Pacific Journal of Tropical Biomedicine 2021;11(6):273-284
CountryChina
Language:Chinese
Abstract: Objective: To investigate the anti-inflammatory effects of the total flavonoids from Saussurea involucrata on lipopolysaccharides (LPS)-stimulated murine RAW264.7 macrophages and explore its underlying mechanism of action. Methods: Total flavonoids from Saussurea involucrata were extracted using chromatographic column method. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The production of nitric oxide was detected by Griess assay and the release of cytokines (IL-10 and TNF-α) and chemokines (MCP-1, MIP-1a, and CCL5/RANTES) was determined by ELISA to evaluate the anti-inflammatory activity of total flavonoids from Saussurea involucrata. Moreover, nuclear translocation of p65, c-Jun, and IRF3 was detected by immunofluorescence microscopy and Western blotting analysis was performed to determine the expression of related proteins. Results: Total flavonoids extracted from Saussurea involucrata were 751.5 mg/g and the content of rutin was 506.5 mg/g. The production of inflammatory mediators including nitric oxide, cytokines, and chemokines was effectively inhibited by total flavonoids from Saussurea involucrata. Meanwhile, total flavonoids also suppressed the nuclear translocation of p65, c-Jun, and IRF3 in LPS-stimulated RAW264.7 cells. The LPS-induced expression of iNOS and COX-2 was remarkably reduced by treatment with total flavonoids from Saussurea involucrata. Moreover, total flavonoids decreased the expression levels of p-IKKa/β, p-TBK1, p-p38, p-ERK, p-JNK, p-p65, p-c-Jun, and p-IRF3 in LPS-exposed RAW264.7 macrophages. Conclusions: Total flavonoids from Saussurea involucrata potentially inhibit the secretion of pro-inflammatory mediators, which may be related to inhibition of p65, c-Jun, and IRF3 signaling pathways in LPS-stimulated RAW264.7 cells.