Ethanol extract of Chondracanthus tenellus (Harvey) Hommersand attenuates lipopolysaccharide-induced inflammatory and oxidative response by blocking the NF-κB, MAPKs, and PI3K/Akt signaling pathways

Da Kwon; Hyesook Lee; Su Hong; Yung Choi; Da Kwon; Hyesook Lee; Su Hong; Yung Choi; Cheol Park; Gi-young Kim; Hee-jae Cha; Suhkmann Kim; Heui-soo Kim; Hye-jin Hwang

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Ethanol extract of Chondracanthus tenellus (Harvey) Hommersand attenuates lipopolysaccharide-induced inflammatory and oxidative response by blocking the NF-κB, MAPKs, and PI3K/Akt signaling pathways

Author: Da KWON ¹ ; Hyesook LEE ¹ ; Su HONG ¹ ; Yung CHOI ¹ ; Da KWON ² ; Hyesook LEE ² ; Su HONG ² ; Yung CHOI ² ; Cheol PARK ³ ; Gi-Young KIM ⁴ ; Hee-Jae CHA ⁵ ; Suhkmann KIM ⁶ ; Heui-Soo KIM ⁷ ; Hye-Jin HWANG ⁸
Author Information

1. Anti-Aging Research Center, Dong-eui University
2. Department of Biochemistry, College of Korean Medicine, Dong-eui University
3. Division of Basic Sciences, College of Liberal Studies, Dong-eui University
4. Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University
5. Department of Parasitology and Genetics, College of Medicine, Kosin University
6. Department of Chemistry, College of Natural Sciences, Pusan National University
7. Department of Biological Sciences, College of Natural Sciences, Pusan National University
8. Department of Food and Nutrition, College of Nursing, Healthcare Sciences and Human Ecology, Dong-eui University
Publication Type:Journal Article
Keywords: Chondracanthus tenellus; Inflammation; NF-κB; RAW 264.7 cells; ROS
From:Asian Pacific Journal of Tropical Biomedicine 2021;11(10):450-459
CountryChina
Language:Chinese
Abstract: Objective: To investigate whether the ethanol extract of Chondracanthus tenellus (Harvey) Hommersand, a type of red algae, could exhibit anti-inflammatory potential in lipopolysaccharide (LPS)-stimulated macrophages. Methods: The ethanol extract of Chondracanthus tenellus was applied to 100 ng/mL LPS-stimulated RAW 264.7 cells, and cell viability, phagocytic ability, levels of pro-inflammatory factors, and the production of reactive oxygen species were measured. To identify the underlying mechanism of the ethanol extract of Chondracanthus tenellus, the expression of inflammation-regulated genes was estimated. Results: The ethanol extract of Chondracanthus tenellus had no cytotoxic effect at concentrations below 300 μg/mL, and reduced the LPS-induced production of inflammatory mediators including nitric oxide (NO) and prostaglandin E 2. Furthermore, the extract markedly suppressed the expression of inducible NO synthase and cyclooxygenase-2, as well as the production of reactive oxygen species. The LPS-induced up-regulation of pro-inflammatory cytokines was attenuated by treatment with the ethanol extract of Chondracanthus tenellus, reducing their extracellular secretion. The Chondracanthus tenellus extract also inhibited LPS-mediated activation of nuclear factor-kappa B (NF-κB). In addition, the phosphorylation of mitogen activated protein kinases (MAPKs) and phosphatidylinositol 3 kinase (PI3K)/Akt was markedly increased by LPS, which was significantly abolished by the Chondracanthus tenellus extract. Conclusions: Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-κB, MAPKs, and PI3K/Akt signaling pathways in LPS stimulated RAW 264.7 macrophages.