Schisandrae Fructus oil-induced elevation in serum triglyceride and lipoprotein concentrations associated with physiologic hepatomegaly in mice
- Author:
Si-Yuan PAN
1
;
Xue-Lan SONG
1
;
Zhao-Heng LIN
1
;
Hai-Chuan TAI
1
;
Si-Yuan PAN
2
;
Qing YU
2
;
Yi ZHANG
2
;
Gan LUO
2
;
Xiao-Yan WANG
2
;
Nan SUN
2
;
Zhu-Sheng CHU
2
;
Yi ZHANG
2
;
Pei-Li ZHU
3
;
Zhi-Ling YU
4
;
Kam-Ming KO
5
,
6
Author Information
- Publication Type:Journal Article
- Keywords: Alanine aminotransferase; Hepatomegaly; Hypertriglyceridemia; Mouse model; Schisandrae Fructus oils; Triglyceride
- From:Asian Pacific Journal of Tropical Biomedicine 2022;12(2):59-68
- CountryChina
- Language:English
- Abstract: Objective: To investigate hypertriglyceridemia and hepatomegaly caused by Schisandrae Sphenantherae Fructus (FSS) and Schisandra chinensis Fructus (FSC) oils in mice. Methods: Mice were orally administered a single dose of Schisandrae Fructus oils. Serum and hepatic triglyceride (TG), triglyceride transfer protein (TTP), apolipoprotein B48 (Apo B48), very-low-density lipoprotein (VLDL), hepatocyte growth factor (HGF), alanine aminotransfease (ALT) and liver index were measured at 6-120 h post-dosing. Results: FSS and FSC oil caused time and dose-dependent increases in serum and hepatic TG levels, with maximum increases in the liver (by 297% and 340%) at 12 h post-dosing and serum (244% and 439%) at 24-h post-dosing, respectively. Schisandrae Fructus oil treatments also elevated the levels of serum TTP by 51% and 63%, Apo B48 by 152% and 425%, and VLDL by 67% and 38% in mice, respectively. FSS and FSC oil treatments also increased liver mass by 53% and 55% and HGF by 106% and 174%, but lowered serum ALT activity by 38% and 22%, respectively. Fenofibrate pre/ co-treatment attenuated the FSS and FSC oil-induced elevation in serum TG levels by 41% and 49% at 48 h post-dosing, respectively, but increased hepatic TG contents (by 38% and 33%, respectively) at 12 h post-dosing. Conclusions: Our findings provide evidence to support the establishment of a novel mouse model of hypertriglyceridemia by oral administration of FSS oil (mainly increasing endogenous TG) and FSC oil (mainly elevating exogenous TG).
