Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress

Kant Sangpairoj; Kant Sangpairoj; Rapeewan Settacomkul; Pornpun Vivithanaporn; Tanapan Siangcham; Krai Meemon; Prasert Sobhon; Nakorn Niamnont; Nilubon Sornkaew; Montakan Tamtin

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Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress

Author: Kant SANGPAIROJ ¹ ; Kant SANGPAIROJ ² ; Rapeewan SETTACOMKUL ³ ; Pornpun VIVITHANAPORN ³ ; Tanapan SIANGCHAM ⁴ ; Krai MEEMON ⁵ ; Prasert SOBHON ⁵ ; Nakorn NIAMNONT ^{6
,

7} ; Nilubon SORNKAEW ^{6
,

7} ; Montakan TAMTIN ⁸
Author Information

1. Division Of Anatomy, Department Of Preclinical Science, Faculty Of Medicine, Thammasat University
2. Thammasat University Research Unit In Neutraceuticals And Food Safety
3. Chakri Naruebodindra Medical Institute, Faculty Of Medicine Ramathibodi Hospital, Mahidol University
4. Faculty Of Allied Health Sciences, Burapha University
5. Department Of Anatomy, Faculty Of Science, Mahidol University
6. Department Of Chemistry, Faculty Of Science, King Mongkut'
7. s University Of Technology Thonburi
8. Department Of Fisheries, Phetcha Buri Coastal Aquaculture Research And Development Center
Publication Type:Journal Article
Keywords: Apoptosis; Autophagy; Endoplasmic reticulum stress; Halymenia durvillei; Hexadecanoic acid; Red algae; Triple-negative breast cancer
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(3):132-140
CountryChina
Language:English
Abstract: Objective: To investigate the effect of the hexane solvent fraction of Halymenia durvillei (HDHE) on triple-negative breast cancer. Methods: The phytochemical profile of HDHE was investigated by GC-MS. The cytotoxicity of HDHE against MDA-MB-231 cells was determined. The apoptotic and autophagic effects of HDHE were analyzed. The expression of molecular markers controlling apoptosis, autophagy, DNA damage, and endoplasmic reticulum (ER) stress was determined. Results: HDHE contains a mixture of fatty acids, mainly hexadecanoic acid. HDHE at a cytotoxic concentration induced apoptotic death of MDA-MB-231 cells through mitochondrial membrane dysfunction, and induction of apoptosis markers, and increased the expression of proteins related to DNA damage response. HDHE also induced the expression of LC-3, a marker of autophagic cell death at a cytotoxic concentration. Moreover, HDHE modulated the expression of ER stress genes. Conclusions: The hexadecanoic acid-enriched extract of Halymenia durvillei promotes apoptosis and autophagy of human triple-negative breast cancer cells. This extract may be further explored as an anticancer agent for triple-negative breast cancer.