Analgesic-like activity of perillyl acetate: In vivo and in silico studies

Renan Braga; Humberto Andrade; Ryldene Cruz; Mayara Maia; Carolina Lima; Allana Duarte; Anderson Santos; André; Miranda; Marcus Scotti; Reinaldo Almeida; Damiã; o Sousa; Reinaldo Almeida; Damiã; o Sousa

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Analgesic-like activity of perillyl acetate: In vivo and in silico studies

Author: Renan BRAGA ^{1
,

2} ; Humberto ANDRADE ^{1
,

2} ; Ryldene CRUZ ^{1
,

2} ; Mayara MAIA ^{1
,

2} ; Carolina LIMA ^{1
,

2} ; Allana DUARTE ^{1
,

2} ; Anderson SANTOS ^{3
,

4} ; André MIRANDA ^{3
,

4} ; Marcus SCOTTI ^{3
,

4} ; Reinaldo ALMEIDA ^{3
,

4} ; Damião SOUSA ^{3
,

4} ; Reinaldo ALMEIDA ^{2
,

5} ; Damião SOUSA ^{2
,

5}
Author Information

1. Postgraduate Program In Natural And Synthetic Bioactive Products, Federal University Of Paraí
2. ba
3. Research Institute For Drugs And Medicines, Federal University Of Paraí
4. ba, Cp 5009
5. Department Of Pharmaceutical Sciences, Federal University Of Paraí
Publication Type:Journal Article
Keywords: Analgesic; Biological products; Essential oil; Monoterpene; Opioid; Pain; Perillyl acetate
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(4):156-163
CountryChina
Language:English
Abstract: Objective: To evaluate the antinociceptive activity of perillyl acetate in mice and in silico simulations. Methods: The vehicle, perillyl acetate (100, 150 and/or 200 mg/kg, i.p.), diazepam (2 mg/kg, i.p.) or morphine (6 mg/kg, i.p.) was administered to mice, respectively. Rotarod test, acetic acid-induced abdominal writhing, formalin-induced nociception, hot plate test, and tail-flick test were performed. Opioid receptors-involvement in perillyl acetate antinociceptive effect was also investigated. Results: Perillyl acetate did not affect the motor coordination of mice. However, it reduced the number of acetic acid-induced abdominal twitches and licking times in the formalin test. There was an increase of latency time in the tail-flick test of 30 and 60 minutes. Pretreatment with naloxone reversed the antinociceptive effect of perillyl acetate (200 mg/kg). In silico analysis demonstrated that perillyl acetate could bind to μ-opioid receptors. Conclusions: Perillyl acetate has antinociceptive effect at the spinal level in animal nociception models, without affecting the locomotor integrity and possibly through μ-opioid receptors. In silico studies have suggested that perillyl acetate can act as a μ-opioid receptor agonist.