Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells
- Author:
Feng WANG
1
;
Suping HAO
1
;
Seon Il JANG
1
;
Byoung Ok CHO
2
,
3
;
Jae Young SHIN
2
,
3
;
Seon Il JANG
2
,
3
;
Feng WANG
4
;
Byoung Ok CHO
5
,
6
Author Information
- Publication Type:Journal Article
- Keywords: Apoptosis; Humulus japonicus; MAPK; Nrf2; Oxidative stress; Parkinson's disease; PC12 cells
- From:Asian Pacific Journal of Tropical Biomedicine 2022;12(5):197-206
- CountryChina
- Language:English
- Abstract: Objective: To explore the possible neuroprotective activities of Humulus japonicus extract against Parkinson's disease (PD) in a cellular model. Methods: PD was modeled in PC12 cells using 6-hydroxydopamine (6-OHDA). The cell activity, intracellular levels of reactive oxygen species (ROS), anti-oxidative and anti-apoptotic effects, and other related indicators and related signaling pathways were evaluated to elucidate the neuroprotective effects of Humulus japonicus extract. Results: Humulus japonicus extract exhibited anti-oxidative and anti-apoptotic effects in 6-OHDA-stimulated PC12 cells. It also reduced oxidative stress-induced ROS accumulation; upregulated antioxidant enzymes, such as glutathione, catalase, heme oxidase-1, and 8-oxguanine glycosylase 1; promoted cell survival by decreasing BAX and increasing Bcl-2 and sirtuin 1 expression via the MAPK and/or Nrf2 signaling pathways. Conclusions: Humulus japonicus extract has antioxidative and anti-apoptotic effects and could be developed as a promising candidate for preventing and treating oxidative stress-related neurodegenerative diseases.
