Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity

Marzieh Rashedinia; Zahra Sabahi; Marzieh Rashedinia; Azita Nasrollahi; Marzieh Shafaghat; Shahrzad Momeni; Forough Iranpak; Jamileh Saberzadeh; Rita Arabsolghar; Forough Iranpak; Rita Arabsolghar

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Syringic acid induces cancer cell death in the presence of Cu (II) ions via pro-oxidant activity

Author: Marzieh RASHEDINIA ¹ ; Zahra SABAHI ¹ ; Marzieh RASHEDINIA ² ; Azita NASROLLAHI ² ; Marzieh SHAFAGHAT ² ; Shahrzad MOMENI ² ; Forough IRANPAK ³ ; Jamileh SABERZADEH ³ ; Rita ARABSOLGHAR ³ ; Forough IRANPAK ⁴ ; Rita ARABSOLGHAR ⁵
Author Information

1. Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences
2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences
3. Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences
4. Department of Biochemistry, Islamic Azad University of Shiraz
5. Department of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences
Publication Type:Journal Article
Keywords: Autophagy; Cancer cell; Copper; Iron; Prooxidant; Syringic acid
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(6):270-278
CountryChina
Language:Chinese
Abstract: Objective: To investigate the effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions. Methods: The antiproliferative effects of syringic acid on HEK 293 and HepG2 cells in the absence and presence of exogenous Cu (II) and Fe (II) ions were examined by MTT assay. Additionally, colony-forming, reactive oxidative species (ROS) generation, apoptosis induction, autophagy, mitochondrial membrane potential, and mitochondrial mass were investigated. Results: At 24 and 72 h, no significant differences were observed in the viability of HepG2 cells between the control and syringic acid + Fe (II) groups. However, exposure of HepG2 cells to syringic acid + Cu (II) for 72 h reduced the cell viability significantly. Furthermore, ROS formation, induction of apoptosis, and autophagic vacuoles were significantly increased in HepG2 cells without marked changes in mitochondrial membrane potential and mitochondrial mass. Moreover, syringic acid + Cu (II) reduced the plating efficiency and surviving fraction significantly. Conclusions: The combination of syringic acid with Cu (II) was toxic to cancer cells and showed pro-oxidant activity. In addition, this combination induced autophagy in cancer cells with less cytotoxic effects on normal cells, which is a potential candidate for the development of novel therapeutics towards cancer.