Dieckol isolated from Eisenia bicyclis extract suppresses RANKL-induced osteoclastogenesis in murine RAW 264.7 cells

Su-hyeon Cho; Hoibin Jeong; Jin Kim; Song-rae Kim; Myeong Seon Jeong; Seonju Park; Miri Choi; Kil-nam Kim; Su-hyeon Cho; Juhee Ahn; Tae-hyung Kwon; Jung-hee Woo; Kil-nam Kim

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Dieckol isolated from Eisenia bicyclis extract suppresses RANKL-induced osteoclastogenesis in murine RAW 264.7 cells

Author: Su-Hyeon CHO ¹ ; Hoibin JEONG ¹ ; Jin KIM ¹ ; Song-Rae KIM ¹ ; Myeong Seon JEONG ¹ ; Seonju PARK ¹ ; Miri CHOI ¹ ; Kil-Nam KIM ¹ ; Su-Hyeon CHO ² ; Juhee AHN ² ; Tae-Hyung KWON ³ ; Jung-Hee WOO ⁴ ; Kil-Nam KIM ⁵
Author Information

1. Chuncheon Center, Korea Basic Science Institute
2. Department of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University
3. Department of Research and Development, Chuncheon Bio-industry Foundation
4. Marine Industry Research Institute for East Se Rim
5. Department of Bio-analysis Science, University of Science & Technology
Publication Type:Journal Article
Keywords: Calcitonin receptor; Dieckol; Eisenia bicyclis; ERK; JNK; NF-κB; NFATc1; Osteoclasts; RANKL; RAW 264.7 cell; TRAP
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(6):262-269
CountryChina
Language:Chinese
Abstract: Objective: To demonstrate the effect of dieckol from Eisenia bicyclis on osteoclastogenesis using RAW 264.7 cells. Methods: Murine macrophage RAW 264.7 cells were subjected to dieckol treatment, followed by treatment with receptor activator of nuclear factor kappa-B ligand (RANKL) to induce osteoclastogenesis. Tartrate-resistant acid phosphatase (TRAP) activity was examined using a TRAP activity kit. Western blotting analysis was conducted to examine the level of osteoclast- related factors, including TRAP and calcitonin receptor (CTR), transcriptional factors, including c-Fos, c-Jun, and nuclear factor of activated T cells cytoplasmic 1 (NFATc1), nuclear factor kappa-B (NF-κB), extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK). Immunofluorescence staining was conducted to examine the expression of c-Fos, c-Jun, and NFATc1. Results: Among the four phlorotannin compounds present in Eisenia bicyclis, dieckol significantly hindered osteoclast differentiation and expression of RANKL-induced TRAP and CTR. In addition, dieckol downregulated the expression levels of c-Fos, c-Jun, NFATc1, ERK, and JNK, and suppressed NF-κB signaling. Conclusions: Dieckol can suppress RANKL-induced osteoclastogenesis. Therefore, it has therapeutic potential in treating osteoclastogenesis- associated diseases.