Hepatoprotective effect of date palm fruit extract against doxorubicin intoxication in Wistar rats: In vivo and in silico studies

Ahmed Fatani; Othman Baothman; Mustafa Zeyadi; Salman Hosawi; Hisham Altayb; Mohamed Abo-golayel; Othman Baothman; Mustafa Zeyadi; Hisham Altayb; Lobna Shash; Huda Abuaraki; Mohamed Abo-golayel

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Hepatoprotective effect of date palm fruit extract against doxorubicin intoxication in Wistar rats: In vivo and in silico studies

Author: Ahmed FATANI ¹ ; Othman BAOTHMAN ¹ ; Mustafa ZEYADI ¹ ; Salman HOSAWI ¹ ; Hisham ALTAYB ¹ ; Mohamed ABO-GOLAYEL ¹ ; Othman BAOTHMAN ² ; Mustafa ZEYADI ² ; Hisham ALTAYB ² ; Lobna SHASH ³ ; Huda ABUARAKI ⁴ ; Mohamed ABO-GOLAYEL ⁵
Author Information

1. Biochemistry Department, Faculty of Science, King Abdulaziz University
2. Microbial Toxicology and Natural Products Center, Faculty of Science, King Abdulaziz University
3. Pathology Department, Faculty of Medicine, Ain Shams University
4. Laboratory Animal Unit, King Fahad Medical Research Centre, King Abdulaziz University
5. Ain Shams University Research Institute, Ain Shams University Hospitals, Ain Shams University
Publication Type:Journal Article
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(8):357-366
CountryChina
Language:English
Abstract: Objective: To investigate the prophylactic efficacy of date palm fruit extract against doxorubicin-induced hepatotoxicity in Wistar albino rats. Methods: The rats were equally and randomly assigned to 6 groups: group 1 (untreated control), group 2 and 3 given daily oral administration of prophylactic aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight, respectively, and group 4, 5 and 6 intraperitoneally injected with doxorubicin at 15 mg/kg on day 30. Rats in group 5 and 6 received daily oral administration of aqueous extract of date palm fruit at 0.75 and 1.5 mg/kg body weight, respectively, for 30 d. The phytochemicals identified by GC-MS analysis were analyzed using in silico study. Antioxidant enzymes, liver enzymatic, biochemical parameters and histopathological analysis were determined to evaluate hepatoprotective activity of date extract. Results: Aqueous extract of date palm fruit significantly mitigated doxorubicin-induced changes in activities of liver enzymes, reduced reactive oxygen species levels, and suppressed lipid peroxidation and DNA damage. Moreover, aqueous extract of date palm fruit reduced doxorubicin-induced hepatic lesions. Molecular docking studies showed that most compounds of aqueous extract of date palm fruit identified via GC-MS had good interaction with proteins of human pregnane X receptor, oxygenase-1, and CYP2C9. Conclusions: The aqueous extract of date palm fruit mitigates doxorubicin-mediated DNA damage and hepatotoxicity, and restores normal liver function and may be a promising agent against the deleterious effects of doxorubicin.