Human Wharton's jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model

Wahyu Widowati; Teresa Wargasetia; Fanny Rahardja; Rimonta Gunanegara; Didik Priyandoko; Marisca Gondokesumo; Ervi Afifah; Cahyaning Wijayanti; Rizal Rizal; Rizal Rizal

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Human Wharton's jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model

Author: Wahyu WIDOWATI ¹ ; Teresa WARGASETIA ¹ ; Fanny RAHARDJA ¹ ; Rimonta GUNANEGARA ¹ ; Didik PRIYANDOKO ² ; Marisca GONDOKESUMO ³ ; Ervi AFIFAH ⁴ ; Cahyaning WIJAYANTI ⁴ ; Rizal RIZAL ⁴ ; Rizal RIZAL ⁵
Author Information

1. Faculty of Medicine, Maranatha Christian University
2. Faculty of Mathematic and Natural Science Education, Indonesia University of Education
3. Faculty of Pharmacy, University of Surabaya, Universitas Surabaya
4. Biomolecular and Biomedical Research Center, Aretha Medika Utama
5. Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering Universitas Indonesia
Publication Type:Journal Article
From:Asian Pacific Journal of Tropical Biomedicine 2022;12(8):343-350
CountryChina
Language:English
Abstract: Objective: To evaluate the potential effect of human Wharton's jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×10 4 /mL, 5×10 5 /mL, 5×10 6 /mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL-18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome.