Genome mining of fungal globin-like enzymes for catalyzing the synthesis of linear terpenes.
10.1016/S1875-5364(22)60192-4
- Author:
Li LIU
1
;
Xiwei CHEN
2
;
Yi ZOU
3
Author Information
1. Lab Teaching & Management Center, Biochemistry and Molecular Biology Laboratory, Chongqing Medical University, Chongqing 401331, China.
2. College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. Electronic address: chenxwimb520@email.swu.edu.cn.
3. College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. Electronic address: zouyi31@swu.edu.cn.
- Publication Type:Journal Article
- Keywords:
C-C double bond cleavage;
Fungi;
Genome mining;
Globin-like enzyme;
Linear terpene
- MeSH:
Terpenes/chemistry*;
Alkyl and Aryl Transferases;
Globins;
Diterpenes
- From:
Chinese Journal of Natural Medicines (English Ed.)
2022;20(10):795-800
- CountryChina
- Language:English
-
Abstract:
Genome mining for the search and discovery of two new globin-like enzymes, TriB fromFusarium poae and TutaA from Schizophyllum commne, are involved in the synthesis of two linear terpenes tricinonoic acid (1) and 2-butenedioic acid (3). Both in vivo heterologous biosynthesis and in vitro biochemical assays showed that these two enzymes catalyzed the C-C double bond cleavage of a cyclic sesquiterpene precursor (-)-germacrene D (7) and a linear diterpene backbone schizostain (2), respectively. Our work presents an unusual formation mechanism of linear terpenes from fungi and expands the functional skills of globin-like enzymes in the synthesis of terpene compounds.
