Determination of ribose and phosphorus contents in Haemophilus influenzae type b capsular polysaccharide by a quantitative NMR method using a single internal standard.
10.1016/S1875-5364(22)60184-5
- Author:
Chun-Jun QIN
1
,
2
;
Jing HU
1
,
2
;
Wei TONG
2
,
3
;
Teng-Teng ZHANG
2
,
3
;
Guang-Zong TIAN
1
,
2
;
Xiao-Peng ZOU
1
,
2
;
Jian-Kai LIU
3
,
4
;
Jian YIN
1
,
5
Author Information
1. Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
2. Jiangnan University-Beijing Minhai Biotechnology Joint Research Center of Carbohydrate-Based Vaccines, Jiangnan University, Wuxi 214122, China.
3. National & Local Joint Engineering Laboratory for Novel Vaccine Development Technology, Beijing Minhai Biotechnology Co., Ltd., Beijing 102600, China
4. Jiangnan University-Beijing Minhai Biotechnology Joint Research Center of Carbohydrate-Based Vaccines, Jiangnan University, Wuxi 214122, China. Electronic address: liujiankai@biominhai.com.
5. Jiangnan University-Beijing Minhai Biotechnology Joint Research Center of Carbohydrate-Based Vaccines, Jiangnan University, Wuxi 214122, China. Electronic address: jianyin@jiangnan.edu.cn.
- Publication Type:Journal Article
- Keywords:
Capsular polysaccharide;
Haemophilus influenzae type b;
Phosphorus content;
Quantitative (1)H-NMR;
Quantitative (31)P-NMR;
Ribose content
- MeSH:
Haemophilus Vaccines;
Haemophilus influenzae type b;
Phosphorus;
Polysaccharides, Bacterial;
Ribose
- From:
Chinese Journal of Natural Medicines (English Ed.)
2022;20(8):633-640
- CountryChina
- Language:English
-
Abstract:
The ribose and phosphorus contents in Haemophilus influenzae type b (Hib) capsular polysaccharide (CPS) are two important chemical indexes for the development and quality control of Hib conjugate vaccine. A quantitative 1H- and 31P-NMR method using a single internal standard was developed for simultaneous determination of ribose and phosphorus contents in Hib CPS. Hexamethylphosphoramide (HMPA) was successfully utilized as an internal standard in quantitative 1H-NMR method for ribose content determination. The ribose and phosphorus contents were found to be affected by the concentration of polysaccharide solution. Thus, 15-20 mg·L-1 was the optimal concentration range of Hib CPS in D2O solution for determination of ribose and phosphorus contents by this method. The ribose and phosphorus contents obtained by the quantitative NMR were consistent with those obtained by traditional chemical methods. In conclusion, this quantitative 1H- and 31P-NMR method using a single internal standard shows good specificity, accuracy and precision, providing a valuable approach for the quality control of Hib glycoconjugate vaccines.
