Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation.
10.1007/s13238-021-00884-1
- Author:
Guang SHI
1
;
Yaofu BAI
1
;
Xiya ZHANG
1
;
Junfeng SU
1
;
Junjie PANG
1
;
Quanyuan HE
1
;
Pengguihang ZENG
2
;
Junjun DING
2
;
Yuanyan XIONG
1
;
Jingran ZHANG
1
;
Jingwen WANG
1
;
Dan LIU
3
;
Wenbin MA
1
;
Junjiu HUANG
4
;
Zhou SONGYANG
5
Author Information
1. MOE Key Laboratory of Gene Function and Regulation, Guangzhou Key Laboratory of Healthy Aging Research, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
2. Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
3. Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
4. MOE Key Laboratory of Gene Function and Regulation, Guangzhou Key Laboratory of Healthy Aging Research, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. hjunjiu@mail.sysu.edu.cn.
5. MOE Key Laboratory of Gene Function and Regulation, Guangzhou Key Laboratory of Healthy Aging Research, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. songyanz@mail.sysu.edu.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords:
Bend5 and Bend4;
chromatin organization;
early development;
embryonic stem cell;
self-renewal and differentiation
- MeSH:
Animals;
Cell Differentiation/genetics*;
Chromatin/metabolism*;
Embryonic Stem Cells;
Germ Cells/metabolism*;
Germ Layers/metabolism*;
Mice
- From:
Protein & Cell
2022;13(10):721-741
- CountryChina
- Language:English
-
Abstract:
Understanding the regulatory networks for germ cell fate specification is necessary to developing strategies for improving the efficiency of germ cell production in vitro. In this study, we developed a coupled screening strategy that took advantage of an arrayed bi-molecular fluorescence complementation (BiFC) platform for protein-protein interaction screens and epiblast-like cell (EpiLC)-induction assays using reporter mouse embryonic stem cells (mESCs). Investigation of candidate interaction partners of core human pluripotent factors OCT4, NANOG, KLF4 and SOX2 in EpiLC differentiation assays identified novel primordial germ cell (PGC)-inducing factors including BEN-domain (BEND/Bend) family members. Through RNA-seq, ChIP-seq, and ATAC-seq analyses, we showed that Bend5 worked together with Bend4 and helped mark chromatin boundaries to promote EpiLC induction in vitro. Our findings suggest that BEND/Bend proteins represent a new family of transcriptional modulators and chromatin boundary factors that participate in gene expression regulation during early germline development.
