Dental stem cell-derived extracellular vesicles transfer miR-330-5p to treat traumatic brain injury by regulating microglia polarization.
10.1038/s41368-022-00191-3
- Author:
Ye LI
1
;
Meng SUN
2
;
Xinxin WANG
1
;
Xiaoyu CAO
2
;
Na LI
2
;
Dandan PEI
3
;
Ang LI
4
Author Information
1. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
2. Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
3. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China. peidandan1986@126.com.
4. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China. drliang@mail.xjtu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Brain Injuries, Traumatic/therapy*;
Chemokines, CXC/metabolism*;
Extracellular Vesicles/metabolism*;
Histocompatibility Antigens/metabolism*;
Histone-Lysine N-Methyltransferase/metabolism*;
Humans;
MicroRNAs/metabolism*;
Microglia/metabolism*;
Rats;
Stem Cells/metabolism*
- From:
International Journal of Oral Science
2022;14(1):44-44
- CountryChina
- Language:English
-
Abstract:
Traumatic brain injury (TBI) contributes to the key causative elements of neurological deficits. However, no effective therapeutics have been developed yet. In our previous work, extracellular vesicles (EVs) secreted by stem cells from human exfoliated deciduous teeth (SHED) offered new insights as potential strategies for functional recovery of TBI. The current study aims to elucidate the mechanism of action, providing novel therapeutic targets for future clinical interventions. With the miRNA array performed and Real-time PCR validated, we revealed the crucial function of miR-330-5p transferred by SHED-derived EVs (SHED-EVs) in regulating microglia, the critical immune modulator in central nervous system. MiR-330-5p targeted Ehmt2 and mediated the transcription of CXCL14 to promote M2 microglia polarization and inhibit M1 polarization. Identified in our in vivo data, SHED-EVs and their effector miR-330-5p alleviated the secretion of inflammatory cytokines and resumed the motor functional recovery of TBI rats. In summary, by transferring miR-330-5p, SHED-EVs favored anti-inflammatory microglia polarization through Ehmt2 mediated CXCL14 transcription in treating traumatic brain injury.
