Whole-Brain Connectome of GABAergic Neurons in the Mouse Zona Incerta.
10.1007/s12264-022-00930-w
- Author:
Yang YANG
1
;
Tao JIANG
2
;
Xueyan JIA
2
;
Jing YUAN
1
;
Xiangning LI
3
;
Hui GONG
4
Author Information
1. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, 430074, China.
2. Research Unit of Multimodal Cross Scale Neural Signal Detection and Imaging, Chinese Academy of Medical Sciences, HUST-Suzhou Institute for Brainsmatics, JITRI, Suzhou, 215123, China.
3. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, 430074, China. lixiangning@mail.hust.edu.cn.
4. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, Wuhan, 430074, China. huigong@mail.hust.edu.cn.
- Publication Type:Journal Article
- Keywords:
GABAergic neurons;
Input circuit;
Output circuit;
Topological connection;
Whole-brain connectome;
Zona incerta
- MeSH:
Animals;
Mice;
Zona Incerta;
GABAergic Neurons;
Connectome
- From:
Neuroscience Bulletin
2022;38(11):1315-1329
- CountryChina
- Language:English
-
Abstract:
The zona incerta (ZI) is involved in various functions and may serve as an integrative node of the circuits for global behavioral modulation. However, the long-range connectivity of different sectors in the mouse ZI has not been comprehensively mapped. Here, we obtained whole-brain images of the input and output connections via fluorescence micro-optical sectioning tomography and viral tracing. The principal regions in the input-output circuits of ZI GABAergic neurons were topologically organized. The 3D distribution of cortical inputs showed rostro-caudal correspondence with different ZI sectors, while the projection fibers from ZI sectors were longitudinally organized in the superior colliculus. Clustering results show that the medial and lateral ZI are two different major functional compartments, and they can be further divided into more subdomains based on projection and input connectivity. This study provides a comprehensive anatomical foundation for understanding how the ZI is involved in integrating different information, conveying motivational states, and modulating global behaviors.
